Indirubin, a Constituent of the Chinese Herbal Medicine Qing-Dai, Attenuates Dextran Sulfate Sodium-induced Murine Colitis

被引：21

作者：

Tokuyasu, Naruo ^{[1
,2
]}

Shomori, Kohei ^{[2
]}

Amano, Kuniki ^{[3
]}

Honjo, Soichiro ^{[1
]}

Sakamoto, Teruhisa ^{[1
]}

Watanabe, Joji ^{[1
]}

Amisaki, Masataka ^{[1
]}

Morimoto, Masaki ^{[1
]}

Uchinaka, Ei ^{[1
]}

Yagyu, Takuki ^{[1
]}

Saito, Hiroaki ^{[1
]}

Ito, Hisao ^{[2
]}

Fujiwara, Yoshiyuki ^{[1
]}

机构：

[1] Tottori Univ, Sch Med, Div Surg Oncol, Dept Surg,Fac Med, Yonago, Tottori 6838503, Japan

[2] Tottori Univ, Sch Med, Div Organ Pathol, Dept Surg,Fac Med, Yonago, Tottori 6838503, Japan

[3] Sky Clin, Hiroshima 7320821, Japan

来源：

YONAGO ACTA MEDICA | 2018年 / 61卷 / 02期

关键词：

Chinese herbal medicine; DSS-induced colitis model; indirubin; inflammatory bowel disease; Qing-Dai; INFLAMMATORY-BOWEL-DISEASE; ULCERATIVE-COLITIS; MONOCLONAL-ANTIBODY; CROHNS-DISEASE; MOUSE COLITIS; MICE; CELLS; INHIBITION; INDUCTION; COMBINATION;

D O I：

10.33160/yam.2018.06.005

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Background Indirubin, a constituent of the Chinese herbal medicine "Qing-Dai," has anti-cancer and anti-inflammatory activities. We aimed to evaluate the efficacy of indirubin for ameliorating colonic inflammation in a mouse model of inflammatory bowel disease. Methods Mice with dextran sulfate sodium (DSS)-induced acute and chronic colitis were treated with indirubin in their diet. Clinical and histologic changes were evaluated. In addition, colon levels of interleukin-6, a critical pro-inflammatory mediator, was detected by enzyme-linked immunosorbent assay. Results In the model of acute colitis, indirubin treatment improved the loss of body weight. Histology of colonic tissue revealed that indirubin treatment improved the histology grading of colitis (P = 0.02), the extent of submucosal fibrosis (P = 0.018), the number of mucosal toluidine blue-positive cells (P = 0.004) and colon length (P = 0.01). In the model of chronic colitis, indirubin treatment had no significant effect on pathologic findings except for colon length (P = 0.003). However, Indirubin administration significantly reduced colon levels of interleukin-6 in the chronic-colitis model (P = 0.001). Conclusion Our study clearly showed that oral intake of indirubin can improve murine DSS-induced colitis (which mimics human inflammatory bowel disease).

引用

页码：128 / 136

页数：9

共 39 条

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