Solid Free-Form Fabrication-Based PCL/HA Scaffolds Fabricated with a Multi-head Deposition System for Bone Tissue Engineering

被引:36
作者
Kim, Jong Young [2 ]
Lee, Tae-Jin [3 ]
Cho, Dong-Woo [2 ,4 ,5 ]
Kim, Byung-Soo [1 ]
机构
[1] Seoul Natl Univ, Sch Chem & Biol Engn, Seoul, South Korea
[2] Andong Natl Univ, Dept Mech Engn, Gyunbuk 760749, South Korea
[3] Hanyang Univ, Dept Bioengn, Seoul 133791, South Korea
[4] POSTECH, Dept Integrat Biosci & Bioengn, Pohang, Gyunbuk, South Korea
[5] POSTECH, Dept Mech Engn, Pohang, Gyunbuk, South Korea
关键词
Bone regeneration; PCL/HA scaffold; multi-head deposition system; solid free-form fabrication; COMPOSITE SCAFFOLDS; STROMAL CELLS; IN-VITRO; DESIGN; POLYCAPROLACTONE; PHOSPHATE; OSTEOGENESIS;
D O I
10.1163/156856209X458380
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
In this study, we fabricated polycaprolactone/hydroxyapatite (PCL/HA) scaffolds with a multi-head deposition system, a solid free-form fabrication technology that was developed in our previous study. The bone regeneration potential of the scaffolds was compared with that of PCL scaffolds fabricated with the same system. The fabricated scaffolds had a pore size of 400 mu m and a porosity of 66.7%. The PCL/HA scaffolds had higher mechanical strength and modulus than the PCL scaffolds. To compare the osteogenic potential, the two types of scaffolds were seeded with rat osteoblasts and cultured in vitro or implanted subcutaneously into athymic mice. The cells cultured on PCL/HA scaffolds expressed higher levels of osteopontin and osteonectin, both of which are osteogenic proteins. The PCL/HA scaffolds resulted in larger bone area and calcium deposition in the implants compared to the PCL scaffolds. (C) Koninklijke Brill NV, Leiden, 2010
引用
收藏
页码:951 / 962
页数:12
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