In vitro plant regeneration and clonal micropropagation of Leontopodium nivale (Ten.) Heut ex Hand.-Mazz. (Asteraceae)

被引：8
作者：
Pace, L. G. [1 ]
Bruno, A. A. [1 ]
Spano, L. [2 ]
机构：
[1] Univ Aquila, Dipartimento Sci Ambientali, I-67010 Coppito, Laquila, Italy
[2] Univ Aquila, Dipartimento Biol Base & Applicata, I-67010 Coppito, Laquila, Italy
来源：
PLANT BIOSYSTEMS | 2009年 / 143卷
关键词：
Edelweiss; endangered species; hydroponics; in vitro regeneration; ALPINUM; EDELWEISS;
D O I：
10.1080/11263500903216214
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
An in vitro micropropagation method was developed for Leontopodium nivale (Ten.) Heut ex Hand.-Mazz., an endemic species of the central Apennines, which is, at present, an endangered plant due to indiscriminate collecting carried out by tourists. Callus induction was obtained from cotyledons of in vitro germinated seeds on medium containing 2,4-dichlorophenoxyacetic acid, and the regeneration of shoots was readily achieved using 6-benzylaminopurine at concentrations ranging from 0.5 to 2.0 mg/L. After rooting on auxin-containing media, plantlets were transferred to hydroponics for acclimation, and then to soil.
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页码：S12 / S16
页数：5
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