Involvement of arabinogalactan proteins in the control of cell proliferation of Cucurbita pepo suspension cultures

被引：14

作者：

Ben Amar, A. ^{[1
,2
]}

Cobanov, P. ^{[1
]}

Ghorbel, A. ^{[2
]}

Mliki, A. ^{[2
]}

Reustle, G. M. ^{[1
]}

机构：

[1] RLP Agrosci GmbH, AlPlanta Inst Plant Res, D-67435 Neustadt, Germany

[2] CBBC, Lab Plant Mol Physiol, Hammam Lif 2050, Tunisia

来源：

BIOLOGIA PLANTARUM | 2010年 / 54卷 / 02期

关键词：

chitinase; embryogenic cultures; extracellular proteins; GLUCOSYL YARIV REAGENT; PLASMA-MEMBRANE; EXPRESSION; WALL; L; EMBRYOGENESIS;

D O I：

10.1007/s10535-010-0055-6

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Arabinogalactan proteins (AGPs) secreted by zucchini squash (Cucurbita pepo L.) cell cultures into the medium are implicated in cell proliferation. Conditioned medium derived from cell suspensions of squash cultivar Dundoo could enhance multiplication rate of slow-growing cell line Cx3005. To examine the role of AGPs, a precipitation assay was performed using Yariv reagent which binds selectively to AGPs. This AGP precipitation as well as proteinase application arrested cell division. However, chitinase treatment successfully increased embryogenic callus mass. A growth promotion was also obtained by arabinogalactan addition to the culture medium. Immunoblotting analysis using the MAC 207 anti-AGP monoclonal antibody showed high AGP expression in Dundoo cell cultures.

引用

页码：321 / 324

页数：4

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