Development and application of Recombinase Polymerase Amplification assay for detection of Bipolaris sorokiniana

被引：16

作者：

Zhao, Wei ^{[1
]}

Chi, Yuan-kai ^{[1
]}

Ye, Meng-di ^{[2
]}

Wang, Tao ^{[1
]}

Xu, A-mei ^{[1
]}

Qi, Ren-de ^{[1
]}

机构：

[1] Anhui Acad Agr Sci, Inst Plant Protect & Agroprod Safety, Hefei, Peoples R China

[2] Anhui Agr Univ, Sch Plant Protect, Hefei, Peoples R China

来源：

CROP PROTECTION | 2021年 / 145卷

基金：

国家重点研发计划;

关键词：

Recombinase polymerase amplification; Bipolaris sorokiniana; Detection; Wheat; CROWN ROT; SPOT BLOTCH; WHEAT; PATHOGENICITY; BARLEY;

D O I：

10.1016/j.cropro.2021.105619

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Bipolaris sorokiniana is a fungal pathogen that caused root rot and spot blotch on wheat. A Recombinase Polymerase Amplification (RPA) assay was developed to detect B. sorokiniana specifically based on the sequences of calmodulin gene sequence. The specificity of RPA assay was tested against 19 fungal organisms associated with wheat, and the detection limit was determined to be 10 pg for B. sorokiniana pure fungal DNA. Further, the RPA assay was capable of detecting B. sorokiniana from artificially inoculated and field collected wheat tissues directly. These results indicate that the RPA assay is rapid and effective method for detection and molecular identification of B. sorokiniana.

引用

页数：5

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