Detecting hypoxia in vitro using 18F-pretargeted IEDDA "click" chemistry in live cells

被引：7

作者：

Allott, Louis ^{[1
,2
]}

Chen, Cen ^{[1
]}

Braga, Marta ^{[1
]}

Leung, Sau Fung Jacob ^{[1
]}

Wang, Ning ^{[1
]}

Barnes, Chris ^{[1
]}

Brickute, Diana ^{[1
]}

Carroll, Laurence ^{[1
,3
]}

Aboagye, Eric O. ^{[1
]}

机构：

[1] Imperial Coll London, Hammersmith Hosp, Dept Surg & Canc, Comprehens Canc Imaging Ctr,Fac Med, Du Cane Rd, London W12 0NN, England

[2] Univ Hull, Fac Hlth Sci, Positron Emiss Tomog Res Ctr, Cottingham Rd, Kingston Upon Hull HU6 7RX, Yorks, England

[3] Johns Hopkins Med Inst, Russell H Morgan Dept Radiol & Radiol Sci, Baltimore, MD 21205 USA

来源：

RSC ADVANCES | 2021年 / 11卷 / 33期

基金：

英国工程与自然科学研究理事会; 英国医学研究理事会;

关键词：

PET; AGENT; FMISO;

D O I：

10.1039/d1ra02482e

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We have exemplified a pretargeted approach to interrogate hypoxia in live cells using radioactive bioorthogonal inverse electron demand Diels-Alder (IEDDA) "click" chemistry. Our novel F-18-tetrazine probe ([F-18]FB-Tz) and 2-nitroimidazole-based TCO targeting molecule (8) showed statistically significant (P < 0.0001) uptake in hypoxic cells (ca. 90 %ID per mg) vs. normoxic cells (<10 %ID per mg) in a 60 min incubation of [F-18]FB-Tz. This is the first time that an intracellularly targeted small-molecule for IEDDA "click" has been used in conjunction with a radioactive reporter molecule in live cells and may be a useful tool with far-reaching applicability for a variety of applications.

引用

页码：20335 / 20341

页数：7

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