Establishment of Epstein-Barr virus-positive human gastric epithelial cell lines

被引：30
作者：
Tajima, M
Komuro, M
Okinaga, K
机构：
[1] Teikyo Univ, Sch Med, Cent Clin Lab, Itabashi Ku, Tokyo 173, Japan
[2] Teikyo Univ, Sch Med, Dept Anat, Itabashi Ku, Tokyo 173, Japan
[3] Teikyo Univ, Sch Med, Dept Surg 2, Itabashi Ku, Tokyo 173, Japan
来源：
JAPANESE JOURNAL OF CANCER RESEARCH | 1998年 / 89卷 / 03期
关键词：
Epstein-Barr virus; gastric carcinoma; cell line; LMP1; EBNA1;
D O I：
10.1111/j.1349-7006.1998.tb00557.x
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
We have established two Epstein-Barr virus (EBV)-positive cell lines, GT38 and GT39, derived from human gastric tissues of two patients bearing gastric carcinoma. Both cell lines mere positive for cytokeratin, an epithelial marker, but not for lymphocyte-related markers, Unlike GT39 cell line, GT38 cells lacked the property of contact inhibition. EBV genome was detected in both cell lines. The cell lines mere positive for latent membrane protein 1, and EBV-determined nuclear antigen 1 (EBNA1). EBNA2 was also detected in GT38. These cell lines should be useful for studying the interaction of EBV with gastric epithelial cells and its role in gastric carcinogenesis.
引用
收藏
页码：262 / 268
页数：7
相关论文
共 35 条
  • [1] POSTTRANSLATIONAL PROCESSING OF AN EPSTEIN-BARR VIRUS-ENCODED MEMBRANE-PROTEIN EXPRESSED IN CELLS TRANSFORMED BY EPSTEIN-BARR-VIRUS
    BAICHWAL, VR
    SUGDEN, B
    [J]. JOURNAL OF VIROLOGY, 1987, 61 (03) : 866 - 875
  • [2] BARRANCO SC, 1983, CANCER RES, V43, P1703
  • [3] EXPRESSION OF THE EPSTEIN-BARR-VIRUS (EBV) LATENT MEMBRANE-PROTEIN IS TIGHTLY REGULATED, INDEPENDENTLY OF EB NUCLEAR ANTIGEN-2 AND OF EBV INTEGRATION OR COPY NUMBER
    CORDIERBUSSAT, M
    CALENDER, A
    VUILLAUME, M
    BORNKAMM, GW
    LENOIR, GM
    [J]. VIRUS RESEARCH, 1993, 27 (01) : 55 - 69
  • [4] EPSTEIN-BARR-VIRUS LATENT MEMBRANE-PROTEIN INHIBITS HUMAN EPITHELIAL-CELL DIFFERENTIATION
    DAWSON, CW
    RICKINSON, AB
    YOUNG, LS
    [J]. NATURE, 1990, 344 (6268) : 777 - 780
  • [5] MORPHOLOGICAL TRANSFORMATION OF HUMAN KERATINOCYTES EXPRESSING THE LMP GENE OF EPSTEIN-BARR-VIRUS
    FAHRAEUS, R
    RYMO, L
    RHIM, JS
    KLEIN, G
    [J]. NATURE, 1990, 345 (6274) : 447 - 449
  • [6] EXPRESSION OF EPSTEIN-BARR VIRUS-ENCODED PROTEINS IN NASOPHARYNGEAL CARCINOMA
    FAHRAEUS, R
    FU, HL
    ERNBERG, I
    FINKE, J
    ROWE, M
    KLEIN, G
    FALK, K
    NILSSON, E
    YADAV, M
    BUSSON, P
    TURSZ, T
    KALLIN, B
    [J]. INTERNATIONAL JOURNAL OF CANCER, 1988, 42 (03) : 329 - 338
  • [7] FUKAYAMA M, 1994, LAB INVEST, V71, P73
  • [8] Epstein-Barr virus infection is an early event in gastric carcinogenesis and is independent of bcl-2 expression and p53 accumulation
    Gulley, ML
    Pulitzer, DR
    Eagan, PA
    Schneider, BG
    [J]. HUMAN PATHOLOGY, 1996, 27 (01) : 20 - 27
  • [9] EPSTEIN-BARR VIRUS-ASSOCIATED GASTRIC ADENOCARCINOMA IN TAIWAN
    HARN, HJ
    CHANG, JY
    WANG, MW
    LO, LI
    LEE, HS
    CHIANG, JH
    LEE, WH
    [J]. HUMAN PATHOLOGY, 1995, 26 (03) : 267 - 271
  • [10] GASTRIC-CARCINOMA - MONOCLONAL EPITHELIAL MALIGNANT-CELLS EXPRESSING EPSTEIN-BARR-VIRUS LATENT INFECTION PROTEIN
    IMAI, S
    KOIZUMI, S
    SUGIURA, M
    TOKUNAGA, M
    UEMURA, Y
    YAMAMOTO, N
    TANAKA, S
    SATO, E
    OSATO, T
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (19) : 9131 - 9135
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