Complex II inactivation is lethal in the nematode Caenorhabditis elegans

被引：19

作者：

Ichimiya, H

Huet, RG

Hartman, P

Amino, H

Kita, K

Ishii, N ^{[1
]}

机构：

[1] Tokai Univ, Sch Med, Dept Mol Life Sci, Kanagawa 2591193, Japan

[2] Texas Christian Univ, Dept Biol, Ft Worth, TX 76129 USA

[3] Univ Tokyo, Grad Sch Med, Dept Biomed Chem, Bunkyo Ku, Tokyo 1130033, Japan

来源：

MITOCHONDRION | 2002年 / 2卷 / 03期

关键词：

electron transport; mitochondria; Caenorhabditis elegans; RNAi; embryogenesis; oxidative stress; complex I; complex II; mev-1; gas-1;

D O I：

10.1016/S1567-7249(02)00069-7

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

RNA-mediated interference (RNAi) was employed to systematically inactivate the four subunits of complex II in the mitochondrial electron transport chain. Embryonic lethality was the predominant result of inactivating three subunits (ceSDHB, ceSDHC, and ceSDHD) when using the soaking method to inactivate RNA. The feeding method was employed to deliver dsRNA from the fourth subunit (ceSDHA) to wild-type, mev-1 (mutated in ceSDHC of complex II), and gas-1 animals (mutated in a complex I gene). Survival was reduced only in the mev-1 genetic background, and in an oxygen-dependent fashion. Collectively, these data provide further evidence that compromised complex II integrity can result in sensitivity to oxidative stress. (C) 2002 Elsevier Science B.V. and Mitochondria Research Society. All rights reserved.

引用

页码：191 / 198

页数：8

共 25 条

[1] The free radical theory of aging matures [J].