Sensory determinants of behavioral dynamics in Drosophila thermotaxis

被引：111

作者：

Klein, Mason ^{[1
]}

Afonso, Bruno ^{[1
,2
]}

Vonner, Ashley J. ^{[1
]}

Hernandez-Nunez, Luis ^{[1
]}

Berck, Matthew ^{[1
]}

Tabone, Christopher J. ^{[1
]}

Kane, Elizabeth A. ^{[1
]}

Pieribone, Vincent A. ^{[3
,4
]}

Nitabach, Michael N. ^{[3
,5
,6
]}

Cardona, Albert ^{[2
]}

Zlatic, Marta ^{[2
]}

Sprecher, Simon G. ^{[2
,7
]}

Gershow, Marc ^{[1
]}

Garrity, Paul A. ^{[8
]}

Samuel, Aravinthan D. T. ^{[1
,2
]}

机构：

[1] Harvard Univ, Dept Phys, Cambridge, MA 02138 USA

[2] Janelia Farm Res Campus, Ashburn, VA 20147 USA

[3] Yale Univ, Sch Med, New Haven, CT 06511 USA

[4] John B Pierce Lab Inc, New Haven, CT 06519 USA

[5] Yale Univ, Sch Med, Dept Genet, New Haven, CT 06520 USA

[6] Yale Univ, Sch Med, Program Cellular Neurosci Neurodegenerat & Repair, New Haven, CT 06520 USA

[7] Univ Fribourg, Dept Biol, Inst Cell & Dev Biol, CH-1700 Fribourg, Switzerland

[8] Brandeis Univ, Dept Biol, Waltham, MA 02435 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2015年 / 112卷 / 02期

基金：

美国国家科学基金会;

关键词：

navigation; reverse correlation; temperature; calcium imaging; voltage imaging; C.-ELEGANS; CAENORHABDITIS-ELEGANS; CHEMOSENSORY NEURONS; MOTION DETECTOR; DECISION-MAKING; TEMPERATURE; BRAIN; LIGHT; MELANOGASTER; CHEMOTAXIS;

D O I：

10.1073/pnas.1416212112

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Complex animal behaviors are built from dynamical relationships between sensory inputs, neuronal activity, and motor outputs in patterns with strategic value. Connecting these patterns illuminates how nervous systems compute behavior. Here, we study Drosophila larva navigation up temperature gradients toward preferred temperatures (positive thermotaxis). By tracking the movements of animals responding to fixed spatial temperature gradients or random temperature fluctuations, we calculate the sensitivity and dynamics of the conversion of thermosensory inputs into motor responses. We discover three thermosensory neurons in each dorsal organ ganglion (DOG) that are required for positive thermotaxis. Random optogenetic stimulation of the DOG thermosensory neurons evokes behavioral patterns that mimic the response to temperature variations. In vivo calcium and voltage imaging reveals that the DOG thermosensory neurons exhibit activity patterns with sensitivity and dynamics matched to the behavioral response. Temporal processing of temperature variations carried out by the DOG thermosensory neurons emerges in distinct motor responses during thermotaxis.

引用

页码：E220 / E229

页数：10

共 45 条

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