Carbapenem inactivation: a very affordable and highly specific method for phenotypic detection of carbapenemase-producing Pseudomonas aeruginosa isolates compared with other methods

被引：22

作者：

Akhi, Mohammad Taghi ^{[1
,2
]}

Khalili, Younes ^{[1
,2
,5
]}

Ghotaslou, Reza ^{[2
]}

Kafil, Hossein Samadi ^{[2
]}

Yousefi, Saber ^{[3
]}

Nagili, Behroz ^{[4
]}

Goli, Hamid Reza ^{[2
]}

机构：

[1] Tabriz Univ Med Sci, Immunol Res Ctr, Tabriz, Iran

[2] Tabriz Univ Med Sci, Dept Microbiol, Fac Med, Tabriz, Iran

[3] Urmia Univ Med Sci, Dept Microbiol & Virol, Fac Med, Orumiyeh, Iran

[4] Tabriz Univ Med Sci, Infect & Trop Dis Res Ctr, Tabriz, Iran

[5] Emam Reza Hosp, Social Secur Org, Orumiyeh, Iran

来源：

JOURNAL OF CHEMOTHERAPY | 2017年 / 29卷 / 03期

关键词：

Pseudomonas aeruginosa; Carbapenem resistance; Carba NP test; Modified Hodge test; Carbapenem inactivation method; MODIFIED HODGE TEST; NP TEST; RAPID DETECTION; ENTEROBACTERIACEAE; IDENTIFICATION; RESISTANCE; IMPACT; ASSAY;

D O I：

10.1080/1120009X.2016.1199506

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

This investigation was undertaken to compare phenotypic and molecular methods for detection of carbapenemaseproducing Pseudomonas aeruginosa. A total of 245 non-duplicated isolates of P. aeruginosa were collected from hospitalized patients. Disc diffusion method was used to identify carbapenem-resistant bacteria. Three phenotypic methods, including Modified Hodge Test (MHT), Modified Carba NP (MCNP) test and Carbapenem Inactivation Method (CIM) were used for investigation of carbapenemase production. In addition, polymerase chain reaction (PCR) was used to detect carbapenemase encoding genes. Of 245 P. aeruginosa isolates investigated, 121 isolates were carbapenem-resistant. Among carbapenem-resistant isolates, 40, 39 and 35 isolates exhibited positive results using MHT, MCNP test and CIM, respectively. PCR indicated the presence of carbapenemase genes in 35 of carbapenem-resistant isolates. MHT showed low sensitivity and specificity for carbapenemase detection among P. aeruginosa isolates in comparison to PCR. CIM was most affordable and highly specific than MCNP test compared with the molecular method.

引用

页码：144 / 149

页数：6

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