Choline dihydrogen phosphate-based deep eutectic solvent: A suitable bioplatform for lipase extraction

被引:13
作者
Alvarez, Maria S. [1 ,2 ]
Rivas, Miguel A. [3 ]
Longo, Maria A. [1 ,2 ]
Deive, Francisco J. [1 ,2 ]
Rodriguez, Ana [1 ]
机构
[1] Univ Vigo, Dept Chem Engn, POB 36310, Vigo, Spain
[2] Univ Vigo, CINTECX, POB 36310, Vigo, Spain
[3] Univ Vigo, Dept Appl Phys, POB 36310, Vigo, Spain
关键词
Dihydrogen phosphate cholinium-based DES; Aqueous biphasic systems; Non-ionic surfactants; Phase equilibrium; Lipolytic enzymes; Partitioning; AQUEOUS BIPHASIC SYSTEMS; IONIC LIQUIDS; NONIONIC SURFACTANTS; AMMONIUM; SEPARATION; TOXICITY; REMOVAL;
D O I
10.1016/j.seppur.2021.118525
中图分类号
TQ [化学工业];
学科分类号
0817 ;
摘要
In this work, we have demonstrated the suitability of a novel Deep Eutectic Solvent (DES) composed of cholinium dihydrogen phosphate and ethylene glycol at a molar ratio of 1:2 (ChDHP:EG) to extract Candida antarctica lipase B from aqueous solutions of non-ionic surfactants Triton X-100 and Tween 80. The binodal curves were firstly explored at temperatures between 298.15 and 333.15 K and the experimental data were correlated by means of empirical models based on exponential and polynomial equations. The tie-lines have been ascertained by means of density and refractive indices measurement, and Othmer-Tobias, Bancroft and Setschenow equations were proposed to correlate these data. The Tween 80-based Aqueous Biphasic System was demonstrated to avoid any deleterious effect on the lipase biocatalytic activity, and more than 80% of the enzyme activity was retained on the DES-rich phase, which proves the suitability of these platforms to be employed in downstream stages in lipase production processes.
引用
收藏
页数:9
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