Chimaeric HBV core particles carrying a defined segment of Puumala hantavirus nucleocapsid protein evoke protective immunity in an animal model

被引：57

作者：

Ulrich, R

Lundkvist, Å

Meisel, H

Koletzki, D

Sjölander, KB

Gelderblom, HR

Borisova, G

Schnitzler, P

Darai, G

Krüger, DH ^{[1
]}

机构：

[1] Humboldt Univ, Charite Med Sch, Inst Med Virol, D-10098 Berlin, Germany

[2] Swedish Inst Infect Dis Control, S-10521 Stockholm, Sweden

[3] Robert Koch Inst, D-13353 Berlin, Germany

[4] Latvian State Univ, Biomed Res & Study Ctr, LV-1067 Riga, Latvia

[5] Univ Heidelberg, Inst Med Virol, D-69120 Heidelberg, Germany

来源：

VACCINE | 1998年 / 16卷 / 2-3期

关键词：

hantavirus; chimaeric virus-like particles; protective immunity;

D O I：

10.1016/S0264-410X(97)00172-2

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Hantaviruses are rodent-born agents which are pathogenic in humans causing haemorrhagic fever with renal syndrome or hantavirus pulmonary syndrome, To induce a protective immunity against a European hantavirus (Puumala) we constructed chimaeric hepatitis B virus (HBV) core particles carrying defined fragments of the Puumala virus nucleocapsid protein, After immunisation of bank voles, the natural host of Puumala virus, with core particles possessing an insertion of the N-terminal part of Puumala virus nucleocapsid protein, four of five animals were protected against subsequent virus challenge, The results show that the major-protective region of the nucleocapsid protein is located between amino acids 1 and 45 and that chimaeric ic HBV core-like particles are useful carriers of foreign protective epitopes. (C) 1997 Elsevier Science Ltd. All rights reserved.

引用

页码：272 / 280

页数：9

共 59 条

[21] NEUTRALIZING HUMAN MONOCLONAL-ANTIBODIES AGAINST PUUMALA VIRUS, CAUSATIVE AGENT OF NEPHROPATHIA-EPIDEMICA - A NOVEL METHOD USING ANTIGEN-COATED MAGNETIC BEADS FOR SPECIFIC B-CELL ISOLATION [J].