Bacterial lipopolysaccharide induces osteoclast formation in RAW 264.7 macrophage cells

被引：130

作者：

Islam, Shamima ^{[1
]}

Hassan, Ferdaus ^{[1
]}

Tumurkhuu, Gantsetseg ^{[1
]}

Dagvadorj, Jargalsaikhan ^{[1
]}

Koide, Naoki ^{[1
]}

Naiki, Yoshikazu ^{[1
]}

Mori, Isamu ^{[1
]}

Yoshida, Tomoaki ^{[1
]}

Yokochi, Takashi ^{[1
]}

机构：

[1] Aichi Med Univ, Sch Med, Dept Microbiol & Immunol, Nagakute, Aichi 4801195, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2007年 / 360卷 / 02期

关键词：

osteoclast; lipopolysaccharide; TNF-alpha; receptor activator of nuclear factor-kappa B ligand (RANKL); Jun-N-terminal kinase 1/2 (JNK1/2); macrophage-colony stimulating factor; tartrate-resistant acid phosphatase (TRAP);

D O I：

10.1016/j.bbrc.2007.06.023

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Lipopolysaccharide (LPS) is a potent bone resorbing factor. The effect of LPS on osteoclast formation was examined by using murine RAW 264.7 macrophage cells. LPS-induced the formation of multinucleated giant cells (MGC) in RAW 264.7 cells 3 days after the exposure. NIGCs were positive for tartrate-resistant acid phosphatase (TRAP) activity. Further, MGC formed resorption pits on calcium-phosphate thin film that is a substrate for osteoclasts. Therefore, LPS was suggested to induce osteoclast formation in RAW 264.7 cells. LPS-induced osteoclast formation was abolished by anti-tumor necrosis factor (TNF)-alpha antibody, but not antibodies to macrophage-colony stimulating factor (M-CSF) and receptor activator of nuclear factor (NF)-kappa B ligand (RANKL). TNF-alpha might play a critical role in LPS-induced osteoclast formation in RAW 264.7 cells. Inhibitors of NF-kappa B and stress activated protein kinase (SAPK/JNK) prevented the LPS-induced osteoclast formation. The detailed mechanism of LPS-induced osteoclast formation is discussed. (C) 2007 Elsevier Inc. All rights reserved.

引用

页码：346 / 351

页数：6

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