Producing marker-free Kalanchoe plants expressing antimicrobial peptide cecropin P1 gene

被引：4

作者：

Zakharchenko, N. S. ^{[1
]}

Lebedeva, A. A. ^{[1
]}

Furs, O. V. ^{[1
]}

Rukavtsova, E. B. ^{[1
]}

Schevchuk, T. V. ^{[1
]}

Rodionov, I. L. ^{[1
]}

Bur'yanov, Y. I. ^{[1
]}

机构：

[1] Russian Acad Sci, Shemyakin & Ovchinnikov Inst Bioorgan Chem, Pushchino Branch, Pushchino 142290, Moscow Oblast, Russia

来源：

RUSSIAN JOURNAL OF PLANT PHYSIOLOGY | 2016年 / 63卷 / 02期

关键词：

Kalanchoe pinnata; P1; agrobacterial transformation; biosafety; cecropin P1; pathogenic microorganisms; transgenic plants; TRANSFORMATION; BACTERIAL; ASSAYS;

D O I：

10.1134/S1021443716020163

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Kalanchoe pinnate (Kalanchoe pinnata L. ) plants with synthetic gene of antimicrobial peptide cecropin P1 (CP1) under the control of promoter 35S RNA of cauliflower mosaic virus (CaMV 35S) were produced. For transformation, a modified binary vector not containing selective genes of tolerance against antibiotics and herbicides was used. Screening of the marker-free transformed plants was conducted on the medium without selective antibiotics by revealing antibacterial activity of plant extracts and cecropin P1. The marker-free plants produced displayed increased resistance against bacterial and fungus phytopathogens, while their extracts were characterized by antimicrobial activity for human and animal pathogens. These plants meet the requirements of biosafety and may be used as producers of cecropin P1 in pharmaceutics.

引用

页码：273 / 282

页数：10

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