Identification of an IKKβ inhibitor for inhibition of inflammation in vivo and in vitro

被引：17

作者：

Chen, Qi ^{[1
,2
]}

Liu, Juan ^{[1
]}

Zhuang, Yuxin ^{[1
]}

Bai, Li-Ping ^{[1
]}

Yuan, Qing ^{[3
]}

Zheng, Silin ^{[2
]}

Liao, Kangsheng ^{[1
]}

Khan, Md Asaduzzaman ^{[4
]}

Wu, Qibiao ^{[1
]}

Luo, Cheng ^{[1
]}

Liu, Liang ^{[1
]}

Wang, Hui ^{[5
]}

Li, Ting ^{[1
]}

机构：

[1] Macau Univ Sci & Technol, State Key Lab Qual Res Chinese Med, Macau Instimro Appl Res Med & Hlth, Ave Wai Long, Taipa, Macao, Peoples R China

[2] Southwest Med Univ, Nursing Dept, Affiliated Hosp, Luzhou 646000, Sichuan, Peoples R China

[3] Southwest Med Univ, Dept Basic Med, Luzhou 646000, Sichuan, Peoples R China

[4] Southwest Med Univ, Res Ctr Preclin Med, Key Lab Epigenet & Oncol, Room 3-319,Zhongshan Rd, Luzhou 646000, Sichuan, Peoples R China

[5] Beijing City Univ, Sch Biol Med, Beijing 100084, Peoples R China

来源：

PHARMACOLOGICAL RESEARCH | 2019年 / 149卷

关键词：

Inflammation; Inhibitor; IKK; NF-KAPPA-B; TNF-ALPHA; AUTOPHAGY; ELLIPTICINE; MACROPHAGES; INTERLEUKIN-6; RELEASE; CELLS; BONE; P62;

D O I：

10.1016/j.phrs.2019.104440

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Targeting on the IKK beta to discover anti-inflammatory drugs has been launched for ten years, due to its predominant role in canonical NF-kappa B signaling. In the current study, we identified a novel IKIC beta inhibitor, ellipticine (ELL), an alkaloid isolated from Ochrosia alpaca and Rauvolfia sandwicensis. We found that ELL reduced the secretion and mRNA expression of TNF-alpha and IL-6 and decreased the protein expression of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) in bone marrow derived macrophages (BMDMs) stimulated with LPS. In coincided with the results, ELL suppressed PGE2 and NO production in BMDMs. Underlying mechanistic study showed that ELL inhibited I kappa B alpha phosphorylation and degradation as well as NF-kappa B nuclear translocation, which was attributed to suppression of IKK alpha/beta activation. Furthermore, kinase assay and binding assay results indicated that ELL inhibited IKK beta activity via directly binding to IKKfi and in turn resulted in suppression of NF-kappa B signaling. To identify the binding sites of ELL on IKK beta, IKK beta(C46A) plasmid was prepared and the kinase assay was performed. The results demonstrated that the inhibitory effect of ELL on IKK beta activity was impaired in the mutation, implying that anti-inflammatory effect of ELL was partially attributed to binding on cysteine 46. Furthermore, ELL up -regulated LC3 II expression and reduced p62 expression, suggesting that autophagy induction contributed to the and-inflammatory effect of ELL as well. In coincided with the in vitro results, ELL increased the survival and antagonized the hypothermia in the mice with LPS-induced septic shock. Consistently, ELL reduced TNF-alpha and IL-6 production in the serum of the mice treated with LPS. Collectively, our study provides evidence that ELL is an IKK beta inhibitor and has potential to be developed as a lead compound for treatment inflammatory diseases in the future.

引用

页数：10

共 40 条

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