Bioprospecting desert plant Bacillus endophytic strains for their potential to enhance plant stress tolerance

被引:82
作者
Bokhari, Ameerah [1 ,7 ]
Essack, Magbubah [2 ]
Lafi, Feras F. [1 ,8 ]
Andres-Barrao, Cristina [1 ]
Jalal, Rewaa [1 ]
Alamoudi, Soha [3 ]
Razali, Rozaimi [2 ]
Alzubaidy, Hanin [1 ]
Shah, Kausar H. [4 ]
Siddique, Shahid [5 ]
Bajic, Vladimir B. [2 ]
Hirt, Heribert [1 ,6 ]
Saad, Maged M. [1 ]
机构
[1] King Abdullah Univ Sci & Technol, Ctr Desert Agr, Thuwal 239556900, Saudi Arabia
[2] King Abdullah Univ Sci & Technol, CBRC, Thuwal 239556900, Saudi Arabia
[3] King Abdulaziz Univ, Dept Biol, Coll Arts & Sci, Rabigh 21589, Saudi Arabia
[4] Bahauddin Zakariya Univ, Inst Pure & Appl Biol, Multan 60800, Pakistan
[5] Univ Calif Davis, Dept Entomol & Nematol, One Shields Ave, Davis, CA 95616 USA
[6] Univ Vienna, Max F Perutz Labs, Dr Bohrgasse 9, A-1030 Vienna, Austria
[7] Adv Res Ctr EXPEC ARC, Explorat & Petr Engn Ctr, Dhahran, Saudi Arabia
[8] Zayed Univ, Coll Nat & Hlth Sci, Abu Dhabi, U Arab Emirates
关键词
GROWTH-PROMOTING RHIZOBACTERIA; DROUGHT STRESS; SOLUBILIZING BACTERIA; CLIMATE-CHANGE; WATER-STRESS; RHIZOSPHERE; RESISTANCE; TOMATO; MECHANISMS; DISEASES;
D O I
10.1038/s41598-019-54685-y
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Plant growth-promoting bacteria (PGPB) are known to increase plant tolerance to several abiotic stresses, specifically those from dry and salty environments. In this study, we examined the endophyte bacterial community of five plant species growing in the Thar desert of Pakistan. Among a total of 368 culturable isolates, 58 Bacillus strains were identified from which the 16 most divergent strains were characterized for salt and heat stress resilience as well as antimicrobial and plant growth-promoting (PGP) activities. When the 16 Bacillus strains were tested on the non-host plant Arabidopsis thaliana, B. cereus PK6-15, B. subtilis PK5-26 and B. circulans PK3-109 significantly enhanced plant growth under salt stress conditions, doubling fresh weight levels when compared to uninoculated plants. B. circulans PK3-15 and PK3-109 did not promote plant growth under normal conditions, but increased plant fresh weight by more than 50% when compared to uninoculated plants under salt stress conditions, suggesting that these salt tolerant Bacillus strains exhibit PGP traits only in the presence of salt. Our data indicate that the collection of 58 plant endophytic Bacillus strains represents an important genomic resource to decipher plant growth promotion at the molecular level.
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页数:13
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