Analysis of synthetic 19-norsteroids trenbolone, tetrahydrogestrinone and gestrinone by gas chromatography-mass spectrometry

被引：19

作者：

Sipoli Marques, Marlice Aparecida ^{[1
]}

Gualberto Pereira, Henrique Marcelo ^{[1
]}

Padilha, Monica Costa ^{[1
]}

de Aquino Neto, Francisco Radler ^{[1
]}

机构：

[1] Univ Fed Rio de Janeiro, Inst Quim, LAB DOP, LADETEC, BR-21949900 Rio De Janeiro, Brazil

来源：

JOURNAL OF CHROMATOGRAPHY A | 2007年 / 1150卷 / 1-2期

关键词：

tetrahydrogestrinone; gestrinone; trenbolone; derivatization;

D O I：

10.1016/j.chroma.2006.08.032

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Tetrahydrogestrinone, gestrinone and trenbolone are synthetic 19-norsteroids with androgenic properties sharing a labile conjugated ketotrienyl motif. Their LC-MS analyses tend to overcome classical derivatization problems, a shortcoming to the use of GC-MS. Therefore, alternative derivatization procedures were evaluated. The procedure with methoxylamine: pyridine followed by TMSImid: MSTFA gave the best results. This is attributed to the stability of the MO-TMS derivatives hindering the formation of artifacts and tautomerism. A full method is presented including SPE, hydrolysis and liquid-liquid extraction. It was possible to confirm the analytes below 2 ng/mL in urine, being the method robust and cost effective also for screening proposes. (C) 2006 Elsevier B.V. All rights reserved.

引用

页码：215 / 225

页数：11

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