Quantitative determination of commercially relevant species in foods by real-time PCR

被引：88

作者：

Laube, Ines ^{[1
]}

Zagon, Jutta ^{[1
]}

Broll, Hermann ^{[1
]}

机构：

[1] Bundeinst Risikobewertung, D-14195 Berlin, Germany

来源：

INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY | 2007年 / 42卷 / 03期

关键词：

cattle; chicken; food; goat; internal positive control; lamb; pig; quantification; TaqMan (TM) PCR; turkey;

D O I：

10.1111/j.1365-2621.2006.01249.x

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Defined samples were examined regarding the proportion of beef, pork, lamb, goat, chicken and turkey utilising TaqMan (TM) PCR. A quantification was performed for determining the proportion of animal species in relation to the total proportion of meat in the food products. The adoption of published real-time PCR systems (International Journal of Food Science and Technology, 2005) permitted a quantitative statement in processed meat products and in canned foods down to a concentration of 0.1%. Different factors influencing the amount of species determined were investigated.

引用

页码：336 / 341

页数：6

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