Allergen stimulates bone marrow CD34+ cells to release IL-5 in vitro;: a mechanism involved in eosinophilic inflammation?

被引:14
作者
Johansson, AK
Sjöstrand, M
Tomaki, M
Samulesson, AM
Lötvall, J
机构
[1] Univ Gothenburg, Inst Internal Med, Dept Resp Med & Allergol, Lung Pharmacol Grp, SE-41346 Gothenburg, Sweden
[2] Tohoku Univ, Sch Med, Dept Resp & Infect Dis, Sendai, Miyagi 980, Japan
[3] Sahlgrens Univ Hosp, Inst Cardiovasc Res, Wallenberg Lab, S-41345 Gothenburg, Sweden
基金
英国科研创新办公室;
关键词
bone marrow; CD34(+) cell; granulocyte macrophage-colony stimulating factor; interleukin-5; interleukin-3;
D O I
10.1111/j.1398-9995.2004.00596.x
中图分类号
R392 [医学免疫学];
学科分类号
100102 ;
摘要
The specific mechanisms that alter bone marrow (BM) eosinophilopoiesis in allergen-induced inflammation are poorly understood. The aims of this study were to evaluate (a) whether the number of BM CD34(+) cells is altered due to allergen sensitization and exposure in vivo and (b) whether BM CD34(+) cells produce and release interleukin (IL)-5, IL-3 and granulocyte macrophage-colony stimulating factor (GM-CSF) after stimulation in vitro. A mouse model of ovalbumin (OVA)-induced airway inflammation was used. Bone marrow CD34(+) cells were cultured in vitro and the cytokine release was measured by enzyme-linked immunosorbent assay. The IL-5-production from CD34(+) cells was confirmed by immunocytochemistry. Airway allergen exposure increased the number of BM CD34(+) cells (P = 0.01). Bone marrow CD34(+) cells produced IL-5 when stimulated with the allergen OVA in vitro, but not IL-3 or GM-CSF. Nonspecific stimulus with calcium ionophore and phorbol-myristate-acetate of BM CD34(+) cells caused release of IL-5, IL-3 and GM-CSF. The induced release of IL-5 was increased in alum-injected vs naive mice (P = 0.02), but was not affected by allergen sensitization and exposure. The release of IL-3 and GM-CSF was increased after allergen sensitization and exposure (P < 0.02). In conclusion, allergen can stimulate BM CD34(+) cells to produce IL-5 protein. It is likely that the CD34(+) cells have autocrine functions and thereby regulate the early stages of BM eosinophilopoiesis induced by airway allergen exposure. Alum, a commonly used adjuvant, enhances the release of IL-5 and may thereby enhance eosinophilopoiesis.
引用
收藏
页码:1080 / 1086
页数:7
相关论文
共 22 条
[1]   DETECTION OF CYTOKINES AND THEIR CELL SOURCES IN BRONCHIAL BIOPSY SPECIMENS FROM ASTHMATIC-PATIENTS - RELATIONSHIP TO ATOPIC STATUS, SYMPTOMS, AND LEVEL OF AIRWAY HYPERRESPONSIVENESS [J].
ACKERMAN, V ;
MARINI, M ;
VITTORI, E ;
BELLINI, A ;
VASSALI, G ;
MATTOLI, S .
CHEST, 1994, 105 (03) :687-696
[2]   Cytokine profiles of BAL T cells and T-cell clones obtained from human asthmatic airways after local allergen challenge [J].
Bodey, KJ ;
Semper, AE ;
Redington, AE ;
Madden, J ;
Teran, LM ;
Holgate, ST ;
Frew, AJ .
ALLERGY, 1999, 54 (10) :1083-1093
[3]   In interleukin-4-deficient mice, alum not only generates T helper 1 responses equivalent to Freund's complete adjuvant, but continues to induce T helper 2 cytokine production [J].
Brewer, JM ;
Conacher, M ;
Satoskar, A ;
Bluethmann, H ;
Alexander, J .
EUROPEAN JOURNAL OF IMMUNOLOGY, 1996, 26 (09) :2062-2066
[4]  
Brewer JM, 1999, J IMMUNOL, V163, P6448
[5]  
CLUTTERBUCK EJ, 1989, BLOOD, V73, P1504
[6]   Rapid increase in bone-marrow eosinophil production and responses to eosinopoietic interleukins triggered by intranasal allergen challenge [J].
Elsas, MICG ;
Joseph, D ;
Elsas, PX ;
Vargaftig, BB .
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY, 1997, 17 (04) :404-413
[7]   Regulation of eosinophilopoiesis in a murine model of asthma [J].
Hogan, MB ;
Weissman, DN ;
Hubbs, AF ;
Gibson, LF ;
Piktel, D ;
Landreth, KS .
JOURNAL OF IMMUNOLOGY, 2003, 171 (05) :2644-2651
[8]   EXPRESSION OF THE POTENT INFLAMMATORY CYTOKINES, GRANULOCYTE-MACROPHAGE-COLONY-STIMULATING FACTOR AND INTERLEUKIN-6 AND INTERLEUKIN-8, IN BRONCHIAL EPITHELIAL-CELLS OF PATIENTS WITH ASTHMA [J].
MARINI, M ;
VITTORI, E ;
HOLLEMBORG, J ;
MATTOLI, S .
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 1992, 89 (05) :1001-1009
[9]   Eosinophil-adhesion-inducing activity produced by antigen-stimulated mononuclear cells involves GM-CSF [J].
Nagata, M ;
Yamamoto, H ;
Tabe, K ;
Sakamoto, Y ;
Matsuo, H .
INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY, 2000, 122 :15-19
[10]   Cytokine and eosinophil responses in the lung, peripheral blood, and bone marrow compartments in a murine model of allergen-induced airways inflammation [J].
Ohkawara, Y ;
Lei, XF ;
Stampfli, MR ;
Marshall, JS ;
Xing, Z ;
Jordana, M .
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY, 1997, 16 (05) :510-520