Metabonomics study of urine from Sprague-Dawley rats exposed to Huang-yao-zi using 1H NMR spectroscopy

被引：59
作者：
Liu, Yanru [1 ,2 ]
Huang, Rongqing [1 ]
Liu, Lijun [3 ]
Peng, Jiangnan [1 ]
Xiao, Bingkun [1 ]
Yang, Jianyun [1 ]
Miao, Zhenchun [3 ]
Huang, Honglin [2 ]
机构：
[1] Acad Mil Med Sci, Inst Radiat Med, Beijing 100850, Peoples R China
[2] Jiangxi Chinese Med Coll, Dept Pharmacochem, Nanchang 330004, Peoples R China
[3] Acad Mil Med Sci, Inst Pharmacol & Toxicol, Beijing 100850, Peoples R China
来源：
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2010年 / 52卷 / 01期
基金：
中国国家自然科学基金;
关键词：
Huang-yao-zi; Metabonomics; H-1; NMR; Liver toxicity; Biomarker; DIOSCOREA-BULBIFERA L; CLERODANE DITERPENOIDS; INDUCED NEPHROTOXICITY; TOXICITY; TRANSFORMATIONS; IDENTIFICATION; SEMISYNTHESIS;
D O I：
10.1016/j.jpba.2009.12.026
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Urinary metabolic perturbations associated with liver toxicity induced by Huang-yao-zi (root of Dioscorea bulifera L.) were studied using nuclear magnetic resonance spectroscopy (H-1 NMR) to determine the correlations between metabonomic profiling and histopathologic/biochemical observations and to discover biomarkers for liver toxicity. Huang-yao-zi with a maximal tolerance dose (MTD) was given to male Sprague-Dawley rats for 72 h followed by metabonomic analysis of urine samples collected at 24 and 72 h. The results revealed that the levels of taurine, creatine, betaine, dimethylglycine (DMG), acetate, glycine were elevated, whereas, the levels of succinate, 2-oxoglutarate, citrate, hippurate and urea were reduced. Partial least square (PLS)-discrimination analysis (DA) of NMR spectra revealed two apparent clusters between control groups and treatment groups, indicating metabolic changes observed in urine samples in response to Huang-yao-zi treatment. In addition, mechanism associated with oxidative injury of hepatic mitochondria was investigated. These results indicated that H-1 NMR-based metabonomics analysis in urine samples may be useful for predicting hepatotoxicity induced by Huang-yao-zi. (C) 2009 Elsevier B.V. All rights reserved.
引用
收藏
页码：136 / 141
页数：6
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