Dietary rescue of altered metabolism gene reveals unexpected Drosophila mating cues

被引:13
作者
Bousquet, Franois [1 ]
Chauvel, Isabelle [1 ]
Flaven-Pouchon, Justin [1 ,2 ]
Farine, Jean-Pierre [1 ]
Ferveur, Jean-Francois [1 ]
机构
[1] Univ Burgundy, Ctr Sci Gout & Alimentat, UMR CNRS 6265, UMR INRA 1324, F-21000 Dijon, France
[2] Univ Valparaiso, Ctr Interdisciplinario Neurociencia Valparaiso, Valparaiso, Chile
关键词
lipid; fitness; hydrocarbon; cis-vaccenyl acetate; POLYUNSATURATED FATTY-ACIDS; SEX-PHEROMONES; DESAT1; MELANOGASTER; TISSUES; FEMALE; REPRODUCTION; DEFICIENCY; EXPRESSION; EMISSION;
D O I
10.1194/jlr.M064683
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
To develop and reproduce, animals need longchain MUFAs and PUFAs. Although some unsaturated FAs (UFAs) can be synthesized by the organism, others must be provided by the diet. The gene, desat1, involved in Drosophila melanogaster UFA metabolism, is necessary for both larval development and for adult sex pheromone communication. We first characterized desat1 expression in larval tissues. Then, we found that larvae in which desat1 expression was knocked down throughout development died during the larval stages when raised on standard food. By contrast pure MUFAs or PUFAs, but not saturated FAs, added to the larval diet rescued animals to adulthood with the best effect being obtained with oleic acid (C18:1). Male and female mating behavior and fertility were affected very differently by preimaginal UFA-rich diet. Adult diet also strongly influenced several aspects of reproduction:flies raised on a C18:1-rich diet showed increased mating performance compared with flies raised on standard adult diet. Therefore, both larval and adult desat1 expression control sex-specific mating signals. A similar nutrigenetics approach may be useful in other metabolic mutants to uncover cryptic effects otherwise masked by severe developmental defects.
引用
收藏
页码:443 / 450
页数:8
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