This research is aimed at investigating the effect of using ethanol (E100) in multi-zone model analysis consisting of multi-combustion chamber zoning cases. The first case considered is a three-zone model that has an unburned zone, burned zone, and transitory zone. The second case model is also three-zone, consisting of an unburned zone and two partitioned burned zones. The burned zone was imagined partitioned into burned zone-1 and burned zone-2 under uneven fuel distribution having different equivalent ratios. The third case is a four-zone model including two regions of burned zone, an unburned zone and a transitory zone, which is unburned burned zone containing a mixture of unburned and burned gases. Arbitrary constants for each of the unburned (CC1) and burned (CC2) Zone leakages in the unburned burned Zone are 0.00025, 0.0005, 0.001, 0.002, 0.005, 0.1 and 0.5. The Mass Fraction Burned (MFB) for zone-1, x1 and burned zone-2, x2 are computed using Partitioned Burnt Zones Ratios (PBZR) of 2:8, 3:7, 4:6, 5:5, 6:4, 7:3 and 8:2. Two equivalent ratios, one for each fuel MFB (phi 1, phi 2), (0.8, 0.6) and (0.6, 0.8) are analyzed using fuel blends of varying percentage. A comparison of values of the three zoning cases is done using peak values from the three-zone models to evaluate the four-zone model. The model was compared with a spark ignition engine (SIE) operating with a premium motor spirit (PMS) serving as baseline. The engine operating conditions were set at an engine speed of 2000 rpm, -35bTDC ignition time, and burn duration at 60 degrees C. The indicated mean effective pressure (IMEP), thermal efficiency (eta), cylinder pressure and emission fraction from the developed models and those of two-zone analysis obtained agreed with literature values. The result showed it is undesirable to have a high volume of burned charge as infiltrate. The three-zone segmented model predicted the highest engine thermal efficiency and peak pressure at mass burn ratio of 7:3. A general reduction in N-2 emission was observed for the three-zone transitional and four-zone models.