RETRACTED: Long non-coding RNA TTN-AS1 promotes the metastasis in breast cancer by epigenetically activating DGCR8 (Retracted Article)

被引：11

作者：

Qiu, P. ^{[1
]}

Dou, Y. ^{[2
]}

Ma, L-Z ^{[2
]}

Tang, X-X ^{[1
]}

Liu, X-L ^{[2
]}

Chen, J-W ^{[2
]}

机构：

[1] Xingtai Peoples Hosp, Dept Med Oncol, Xingtai, Peoples R China

[2] Xingtai Peoples Hosp, Dept Pathol, Xingtai, Peoples R China

来源：

EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES | 2019年 / 23卷 / 24期

关键词：

Long non-coding RNA; TTN-AS1; BC; DGCR8; PROLIFERATION; PROGRESSION; STATISTICS;

D O I：

10.26355/eurrev_201912_19787

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

OBJECTIVE: Breast cancer (BC) is one of the most common fatal cancers. Recent studies have identified the vital roles of long non-coding RNAs (lncRNAs) in the development and progression of BC. This research aimed to investigate the underlying mechanisms of lncRNA TTN-AS1 in the metastasis of BC. PATIENTS AND METHODS: TTN-AS1 expression of tissues was detected by Real Time-quantitative Polymerase Chain Reaction (RT-qPCR) in 50 BC patients. Wound healing assay and transwell assay were used to observe the phenotypic alteration of BC cells after knockdown or overexpression of TTN-AS1. Moreover. RT-qPCR and Western blot assay were performed to discover the potential targets of TTN-AS1 in BC. RESULTS: TTN-AS1 expression in BC samples was significantly higher than that of the adjacent tissues. Besides, the migration and invasion of BC cells were markedly inhibited after TTN-AS1 was silenced, while promoted after TTN-AS1 overexpression. In addition, a remarkable decrease of DGCR8 was observed after TTN-AS1 was inhibited in BC cells. while DGCR8 was upregulated after overexpression of TTN-AS1. Furthermore, DGCR8 expression showed significant enhancement in BC tissues and was positively associated with TTN-AS1 level. CONCLUSIONS: Our study uncovered a new oncogene in BC and suggested that TTN-AS1 could enhance BC cell migration and invasion via sponging DGCR8, which provided a novel therapeutic target for the treatment of breast cancer.

引用

页码：10835 / 10841

页数：7

共 17 条

[1]

[Anonymous], J GENE MED

[2] DGCR8 is essential for tumor progression following PTEN loss in the prostate [J].

Belair, Cassandra D. ;

Paikari, Alireza ;

Moltzahn, Felix ;

Shenoy, Archana ;

Yau, Christina ;

Dall'Era, Marc ;

Simko, Jeff ;

Benz, Christopher ;

Blelloch, Robert .

EMBO REPORTS, 2015, 16 (09) :1219-1232

[3] Long non-coding RNA TTN-AS1 promotes cell growth and metastasis in cervical cancer via miR-573/E2F3 [J].

Chen, Ping ;

Wang, Rui ;

Yue, Qingfen ;

Hao, Mengmeng .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2018, 503 (04) :2956-2962

[4] Long non-coding RNA HOXA-AS2 promotes proliferation and invasion of breast cancer by acting as a miR-520c-3p sponge [J].

Fang, Yu ;

Wang, Jingxuan ;

Wu, Feng ;

Song, Ying ;

Zhao, Shu ;

Zhang, Qingyuan .

ONCOTARGET, 2017, 8 (28) :46090-46103

[5] Silencing the Double-Stranded RNA Binding Protein DGCR8 Inhibits Ovarian Cancer Cell Proliferation, Migration, and Invasion [J].

Guo, Yuqi ;

Tian, Peng ;

Yang, Chuanhe ;

Liang, Zhibing ;

Li, Min ;

Sims, Michelle ;

Lu, Lu ;

Zhang, Zhan ;

Li, Hongwei ;

Pfeffer, Lawrence M. ;

Yue, Junming .

PHARMACEUTICAL RESEARCH, 2015, 32 (03) :769-778

[6] Long non-coding RNA TP73-AS1 promotes colorectal cancer proliferation by acting as a ceRNA for miR-103 to regulate PTEN expression [J].

Jia, Zeming ;

Peng, Jian ;

Yang, Zhi ;

Chen, Jie ;

Liu, Ling ;

Luo, Dongren ;

He, Panxiang .

GENE, 2019, 685 :222-229

[7] Long Non-Coding RNA TUG1 Promotes Proliferation and Inhibits Apoptosis of Osteosarcoma Cells by Sponging miR-132-3p and Upregulating SOX4 Expression [J].

Li, Gang ;

Liu, Keyu ;

Du, Xinhui .

YONSEI MEDICAL JOURNAL, 2018, 59 (02) :226-235

[8] Functional Role of a Novel Long Noncoding RNA TTN-AS1 in Esophageal Squamous Cell Carcinoma Progression and Metastasis [J].

Lin, Chenyu ;

Zhang, Shengnan ;

Wang, Ying ;

Wang, Yuanshu ;

Nice, Edouard ;

Guo, Changying ;

Zhang, Erhao ;

Yu, Liting ;

Li, Mengwei ;

Liu, Chen ;

Hu, Lirong ;

Hao, Jingchao ;

Qi, Weiyan ;

Xu, Hanmei .

CLINICAL CANCER RESEARCH, 2018, 24 (02) :486-498

[9] lncRNA GAS5 Reverses EMT and Tumor Stem Cell-Mediated Gemcitabine Resistance and Metastasis by Targeting miR-221/SOCS3 in Pancreatic Cancer [J].

Liu, Bingyan ;

Wu, Shaoqiu ;

Ma, Jun ;

Yan, Shuo ;

Xiao, Zhengguang ;

Wan, Linhuang ;

Zhang, Feng ;

Shang, Mingyi ;

Mao, Aiwu .

MOLECULAR THERAPY-NUCLEIC ACIDS, 2018, 13 :472-482

[10]

Liu XM, 2018, EUR REV MED PHARMACO, V22, P8749, DOI 10.26355/eurrev_201812_16640

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