The ribosomal peptidyl transferase

被引：120
作者：
Beringer, Malte [1 ]
Rodnina, Marina V. [1 ]
机构：
[1] Univ Witten Herdecke, Inst Phys Biochem, D-58448 Witten, Germany
来源：
MOLECULAR CELL | 2007年 / 26卷 / 03期
关键词：
PEPTIDYLTRANSFERASE ACTIVE-SITE; SUBSTRATE-ASSISTED CATALYSIS; AMINOACYL-TRANSFER-RNA; BOND FORMATION; CRYSTAL-STRUCTURE; BASE-PAIR; STRUCTURAL INSIGHTS; PUROMYCIN REACTION; MECHANISM; INTERMEDIATE;
D O I：
10.1016/j.molcel.2007.03.015
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Peptide bond formation on the ribosome takes place in an active site composed of RNA. Recent progress of structural, biochemical, and computational approaches has provided a fairly detailed picture of the catalytic mechanism of the reaction. The ribosome accelerates peptide bond formation by lowering the activation entropy of the reaction due to positioning the two substrates, ordering water in the active site, and providing an electrostatic network that stabilizes the reaction intermediates. Proton transfer during the reaction appears to be promoted by a concerted proton shuttle mechanism that involves ribose hydroxyl groups on the tRNA substrate.
引用
收藏
页码：311 / 321
页数：11
相关论文
共 62 条
  • [1] Crystal structure of a self-splicing group I intron with both exons
    Adams, PL
    Stahley, MR
    Kosek, AB
    Wang, JM
    Strobel, SA
    [J]. NATURE, 2004, 430 (6995) : 45 - 50
  • [2] The complete atomic structure of the large ribosomal subunit at 2.4 Å resolution
    Ban, N
    Nissen, P
    Hansen, J
    Moore, PB
    Steitz, TA
    [J]. SCIENCE, 2000, 289 (5481) : 905 - 920
  • [3] BARTA A, 1996, RIBOSOMAL RNA GROUP, P35
  • [4] Structural basis of the ribosomal machinery for peptide bond formation, translocation, and nascent chain progression
    Bashan, A
    Agmon, I
    Zarivach, R
    Schluenzen, F
    Harms, J
    Berisio, R
    Bartels, H
    Franceschi, F
    Auerbach, T
    Hansen, HAS
    Kossoy, E
    Kessler, M
    Yonath, A
    [J]. MOLECULAR CELL, 2003, 11 (01) : 91 - 102
  • [5] The A2453-C2499 wobble base pair in Escherichia coli 23S ribosomal RNA is responsible for pH sensitivity of the peptidyltransferase active site conformation
    Bayfield, MA
    Thompson, J
    Dahlberg, AE
    [J]. NUCLEIC ACIDS RESEARCH, 2004, 32 (18) : 5512 - 5518
  • [6] A conformational change in the ribosomal peptidyl transferase center upon active/inactive transition
    Bayfield, MA
    Dahlberg, AE
    Schulmeister, U
    Dorner, S
    Barta, A
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (18) : 10096 - 10101
  • [7] Essential mechanisms in the catalysis of peptide bond formation on the ribosome
    Beringer, M
    Bruell, C
    Xiong, LQ
    Pfister, P
    Bieling, P
    Katunin, VI
    Mankin, AS
    Böttger, EC
    Rodnina, MV
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2005, 280 (43) : 36065 - 36072
  • [8] The G2447A mutation does not affect ionization of a ribosomal group taking part in peptide bond formation
    Beringer, M
    Adio, S
    Wintermeyer, W
    Rodnina, M
    [J]. RNA, 2003, 9 (08) : 919 - 922
  • [9] Peptide bond formation does not involve acid-base catalysis by ribosomal residues
    Bieling, P
    Beringer, M
    Adio, S
    Rodnina, MV
    [J]. NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2006, 13 (05) : 423 - 428
  • [10] The interaction between C75 of tRNA and the A loop of the ribosome stimulates peptidyl transferase activity
    Brunelle, JL
    Youngman, EM
    Sharma, D
    Green, R
    [J]. RNA, 2006, 12 (01) : 33 - 39
1234567