Focus: The interface between data collection and data processing in cryo-EM

被引：146

作者：

Biyani, Nikhil ^{[1
]}

Righetto, Ricardo D. ^{[1
]}

McLeod, Robert ^{[1
]}

Caujolle-Bert, Daniel ^{[2
]}

Castano-Diez, Daniel ^{[2
]}

Goldie, Kenneth N. ^{[1
]}

Stahlberg, Henning ^{[1
]}

机构：

[1] Univ Basel, Biozentrum, Ctr Cellular Imaging & NanoAnalyt C CINA, Basel, Switzerland

[2] Univ Basel, C CINA, Biozentrum, BioEM Lab, Basel, Switzerland

来源：

JOURNAL OF STRUCTURAL BIOLOGY | 2017年 / 198卷 / 02期

基金：

瑞士国家科学基金会;

关键词：

Cryo-EM; High throughput image processing; Automated data collection; Drift correction; BEAM-INDUCED MOTION; ELECTRON; ALIGNMENT; RECONSTRUCTION; IMPLEMENTATION; ACQUISITION; TOMOGRAPHY; REFINEMENT; MICROSCOPY; FREALIGN;

D O I：

10.1016/j.jsb.2017.03.007

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We present a new software package called Focus that interfaces cryo-transmission electron microscopy (cryo-EM) data collection with computer image processing. Focus creates a user-friendly environment to import and manage data recorded by direct electron detectors and perform elemental image processing tasks in a high-throughput manner while new data is being acquired at the microscope. It provides the functionality required to remotely monitor the progress of data collection and data processing, which is essential now that automation in cryo-EM allows a steady flow of images of single particles, two-dimensional crystals, or electron tomography data to be recorded in overnight sessions. The rapid detection of any errors that may occur greatly increases the productivity of recording sessions at the electron microscope. (C) 2017 The Author(s). Published by Elsevier Inc.

引用

页码：124 / 133

页数：10

共 44 条

[1] Dynamo: A flexible, user-friendly development tool for subtomogram averaging of cryo-EM data in high-performance computing environments [J].