The Yersinia Ser/Thr protein kinase YpkA/YopO directly interacts with the small GTPases RhoA and Rac-1

被引：86

作者：

Barz, C ^{[1
]}

Abahji, TN ^{[1
]}

Trülzsch, K ^{[1
]}

Heesemann, J ^{[1
]}

机构：

[1] Max Von Pettenkofer Inst Hyg & Med Mikrobiol, D-80336 Munich, Germany

来源：

FEBS LETTERS | 2000年 / 482卷 / 1-2期

关键词：

Rho; Rac; Yop; YopO; YpkA; Yersinia;

D O I：

10.1016/S0014-5793(00)02045-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Pathogenic bacteria of the genus Yersinia counteract host defense by interfering with eukaryotic signal transduction pathways. YpkA of Yersinia pseudotuberculosis shares significant homology with eukaryotic Ser/Thr protein kinases, is translocated into the host cell and has been shown to be an essential virulence factor in a mouse infection model. In this study, we identify the small GTPases RhoA and Rac-1 as eukaryotic binding partners of YpkA and its homolog YopO of Yersinia enterocolitica. We demonstrate that the interaction is independent of phosphorylation of YpkA and nucleotide loading state of the GTPases. The interaction with RhoA and Rac-1 might provide an important clue to how YpkA interferes with eukaryotic signaling on a molecular level, (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：139 / 143

页数：5

共 30 条

[1] The tranquilizing injection of Yersinia proteins:: A pathogen's strategy to resist host defense [J].

Aepfelbacher, M ;

Zumbihl, R ;

Ruckdeschel, K ;

Jacobi, CA ;

Barz, C ;

Heesemann, J .

BIOLOGICAL CHEMISTRY, 1999, 380 (7-8) :795-802

[2] Rho proteins: Targets for bacterial toxins [J].

Aktories, K .

TRENDS IN MICROBIOLOGY, 1997, 5 (07) :282-288

[3] Effectors for the Rho GTPases [J].

Aspenström, P .

CURRENT OPINION IN CELL BIOLOGY, 1999, 11 (01) :95-102

[4] Identification of p130(Cas) as a substrate of Yersinia YopH (Yop51), a bacterial protein tyrosine phosphatase that translocates into mammalian cells and targets focal adhesions [J].

Black, DS ;

Bliska, JB .

EMBO JOURNAL, 1997, 16 (10) :2730-2744

[5] Status of YopM and YopN in the Yersinia Yop virulon: YopM of Y-enterocolitica is internalized inside the cytosol of PU5-1.8 macrophages by the YopB, D, N delivery apparatus [J].

Boland, A ;

Sory, MP ;

Iriarte, M ;

Kerbourch, C ;

Wattiau, P ;

Cornelis, GR .

EMBO JOURNAL, 1996, 15 (19) :5191-5201

[6] Role of YopP in suppression of tumor necrosis factor alpha release by macrophages during Yersinia infection [J].

Boland, A ;

Cornelis, GR .

INFECTION AND IMMUNITY, 1998, 66 (05) :1878-1884

[7] The virulence plasmid of Yersinia, an antihost genome [J].

Cornelis, GR ;

Boland, A ;

Boyd, AP ;

Geuijen, C ;

Iriarte, M ;

Neyt, C ;

Sory, MP ;

Stainier, I .

MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 1998, 62 (04) :1315-+

[8] The Yersinia Yop virulon: A bacterial system for subverting eukaryotic cells [J].

Cornelis, GR ;

WolfWatz, H .

MOLECULAR MICROBIOLOGY, 1997, 23 (05) :861-867

[9] A Salmonella protein antagonizes Rac-1 and Cdc42 to mediate host-cell recovery after bacterial invasion [J].

Fu, YX ;

Galán, JE .

NATURE, 1999, 401 (6750) :293-297

[10] A SECRETED PROTEIN-KINASE OF YERSINIA-PSEUDOTUBERCULOSIS IS AN INDISPENSABLE VIRULENCE DETERMINANT [J].

GALYOV, EE ;

HAKANSSON, S ;

FORSBERG, A ;

WOLFWATZ, H .

NATURE, 1993, 361 (6414) :730-732

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