Immobilization of acylase on porous silica beads: preparation and thermal activation studies

被引：12

作者：

He, F ^{[1
]}

Zhuo, RX ^{[1
]}

Liu, LJ ^{[1
]}

Xu, MY ^{[1
]}

机构：

[1] Wuhan Univ, Dept Chem, Lab Biomed Polymer Mat, Minist Educ China, Wuhan 430072, Peoples R China

来源：

REACTIVE & FUNCTIONAL POLYMERS | 2000年 / 45卷 / 01期

关键词：

acylase; immobilization; thermal activation; thermal stability;

D O I：

10.1016/S1381-5148(00)00008-0

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Acylase (EC 3.5.1.14) was immobilized on porous silica beads by cross linking with glutaraldehyde. The factors affecting the activity of the immobilized acylase, such as pH of the immobilization reaction, concentration of glutaraldehyde and amount of acylase were studied. We also studied the thermal properties of immobilized acylase. Thermal activation of this immobilized acylase was found at a temperature range of 50-90 degrees C. The durability of the immobilized acylase on heating was greatly improved. Even after incubation at 70 degrees C for 6 h, the activity of immobilized acylase was higher than that of the unincubated one. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：29 / 33

页数：5