Accurate and sensitive total genomic DNA methylation analysis from sub-nanogram input with embedded SERS nanotags

被引：41

作者：

Wang, Yuling ^{[1
]}

Wee, Eugene J. H. ^{[1
]}

Trau, Matt ^{[1
,2
]}

机构：

[1] AIBN, Ctr Personalized NanoMed, Corner Coll & Cooper Rd Bldg 75, Brisbane, Qld 4072, Australia

[2] Univ Queensland, Sch Chem & Mol Biosci, Brisbane, Qld 4072, Australia

来源：

CHEMICAL COMMUNICATIONS | 2016年 / 52卷 / 17期

基金：

澳大利亚研究理事会;

关键词：

ENHANCED RAMAN-SPECTROSCOPY; LABEL-FREE DETECTION; NANOPARTICLES; SCATTERING; CANCER; 5-METHYLCYTOSINE; HYPOMETHYLATION; MICROSCOPY; DESIGN;

D O I：

10.1039/c6cc00547k

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Sensitive and accurate total genomic DNA methylation analysis was demonstrated by surface-enhanced Raman scattering (SERS) via embedded internal SERS nanotags. The assay was sensitive to 0.2 ng input DNA while differentiating as low as 6.25% changes in DNA methylation. The method could also successfully differentiate cells before and after de-methylating drug-treatment and between tumor and normal biopsies.

引用

页码：3560 / 3563

页数：4

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