Accurate and sensitive total genomic DNA methylation analysis from sub-nanogram input with embedded SERS nanotags

被引：40

作者：

Wang, Yuling ^{[1
]}

Wee, Eugene J. H. ^{[1
]}

Trau, Matt ^{[1
,2
]}

机构：

[1] AIBN, Ctr Personalized NanoMed, Corner Coll & Cooper Rd Bldg 75, Brisbane, Qld 4072, Australia

[2] Univ Queensland, Sch Chem & Mol Biosci, Brisbane, Qld 4072, Australia

来源：

CHEMICAL COMMUNICATIONS | 2016年 / 52卷 / 17期

基金：

澳大利亚研究理事会;

关键词：

ENHANCED RAMAN-SPECTROSCOPY; LABEL-FREE DETECTION; NANOPARTICLES; SCATTERING; CANCER; 5-METHYLCYTOSINE; HYPOMETHYLATION; MICROSCOPY; DESIGN;

D O I：

10.1039/c6cc00547k

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Sensitive and accurate total genomic DNA methylation analysis was demonstrated by surface-enhanced Raman scattering (SERS) via embedded internal SERS nanotags. The assay was sensitive to 0.2 ng input DNA while differentiating as low as 6.25% changes in DNA methylation. The method could also successfully differentiate cells before and after de-methylating drug-treatment and between tumor and normal biopsies.

引用

页码：3560 / 3563

页数：4

共 39 条

[1] Epigenetic gene silencing in cancer - a mechanism for early oncogenic pathway addiction? [J].

Baylin, SB ;

Ohm, JE .

NATURE REVIEWS CANCER, 2006, 6 (02) :107-116

[2] Genome-wide DNA methylation profiling using Infinium® assay [J].

Bibikova, Marina ;

Le, Jennie ;

Barnes, Bret ;

Saedinia-Melnyk, Shadi ;

Zhou, Lixin ;

Shen, Richard ;

Gunderson, Kevin L. .

EPIGENOMICS, 2009, 1 (01) :177-200

[3] Single-Molecule Surface-Enhanced Raman Spectroscopy of Nonresonant Molecules [J].

Blackie, Evan J. ;

Le Ru, Eric C. ;

Etchegoin, Pablo G. .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2009, 131 (40) :14466-14472

[4]

CLARK SJ, 1994, NUCLEIC ACIDS RES, V22, P2990, DOI 10.1093/nar/22.15.2990

[5] Bisulfite-free analysis of 5MeC-binding proteins and locus-specific methylation density using a microparticle-based flow cytometry assay [J].

Corrie, Simon R. ;

Sova, Pavel ;

Feng, Qinghua ;

Blair, Tiffany ;

Kiviat, Nancy B. ;

Trau, Matt .

ANALYST, 2011, 136 (04) :688-691

[6] DNA hypomethylation in cancer cells [J].

Ehrlich, Melanie .

EPIGENOMICS, 2009, 1 (02) :239-259

[7] Direct observation of size-dependent optical enhancement in single metal nanoparticles [J].

Emory, SR ;

Haskins, WE ;

Nie, SM .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1998, 120 (31) :8009-8010

[8] DNA methylation: A profile of methods and applications [J].

Fraga, ME ;

Esteller, M .

BIOTECHNIQUES, 2002, 33 (03) :632-+

[9] Global DNA Hypomethylation in Peripheral Blood Mononuclear Cells as a Biomarker of Cancer Risk [J].

Friso, Simonetta ;

Udali, Silvia ;

Guarini, Patrizia ;

Pellegrini, Camilla ;

Pattini, Patrizia ;

Moruzzi, Sara ;

Girelli, Domenico ;

Pizzolo, Francesca ;

Martinelli, Nicola ;

Corrocher, Roberto ;

Olivieri, Oliviero ;

Choi, Sang-Woon .

CANCER EPIDEMIOLOGY BIOMARKERS & PREVENTION, 2013, 22 (03) :348-355

[10] A GENOMIC SEQUENCING PROTOCOL THAT YIELDS A POSITIVE DISPLAY OF 5-METHYLCYTOSINE RESIDUES IN INDIVIDUAL DNA STRANDS [J].

FROMMER, M ;

MCDONALD, LE ;

MILLAR, DS ;

COLLIS, CM ;

WATT, F ;

GRIGG, GW ;

MOLLOY, PL ;

PAUL, CL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (05) :1827-1831

← 1 2 3 4 →