The yeast Snm1 protein is a DNA 5′-exonuclease

被引：61

作者：

Li, XR ^{[1
]}

Hejna, J ^{[1
]}

Moses, RE ^{[1
]}

机构：

[1] Oregon Hlth Sci Univ, Portland, OR 97239 USA

来源：

DNA REPAIR | 2005年 / 4卷 / 02期

关键词：

DNA repair; interstrand cross-links; SNM1; exonuclease;

D O I：

10.1016/j.dnarep.2004.08.012

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Interstrand cross-links (ICL) in DNA arise from bifunctional alkylating agents, including nitrogen mustards, mitomycin C and psoralens. Such adducts prevent normal transcription or replication and are mutagenic. Therefore, cellular mechanisms for removing ICL damage are needed to maintain genome stability. Normal ICL repair requires the action of a number of genes, some specific for such damage. The yeast Snm1 protein is one such protein, but its function has been unknown. Incision for ICL repair is normal in mutants lacking Snm1, so it appears to act after the earliest steps. We have used recombinant SNM1 constructs in an Escherichia coli (E. coli) expression system to demonstrate that the yeast gene encodes a 5'-exonuclease. The exonuclease activity is required for Snm1 to be functional in ICL repair. (C) 2004 Elsevier B.V. All rights reserved.

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页码：163 / 170

页数：8

相关论文

共 22 条

[1] Artemis: guarding small children and, now, the genome [J].

Brandt, VL ;

Roth, DB .

JOURNAL OF CLINICAL INVESTIGATION, 2003, 111 (03) :315-316

[2] The Fanconi anaemia BRCA pathway [J].

D'Andrea, AD ;

Grompe, M .

NATURE REVIEWS CANCER, 2003, 3 (01) :23-34

[3] Repair of DNA interstrand cross-links [J].

Dronkert, MLG ;

Kanaar, R .

MUTATION RESEARCH-DNA REPAIR, 2001, 486 (04) :217-247

[4] Disruption of mouse SNM1 causes increased sensitivity to the DNA interstrand cross-linking agent mitomycin C [J].

Dronkert, MLG ;

de Wit, J ;

Boeve, M ;

Vasconcelos, ML ;

van Steeg, H ;

Tan, TLR ;

Hoeijmakers, JHJ ;

Kanaar, R .

MOLECULAR AND CELLULAR BIOLOGY, 2000, 20 (13) :4553-4561

[5] Saccharomyces cerevisiae lacking Snm1, Rev3 or Rad51 have a normal S-phase but arrest permanently in G2 after cisplatin treatment [J].

Grossmann, KF ;

Ward, AM ;

Moses, RE .

MUTATION RESEARCH-DNA REPAIR, 2000, 461 (01) :1-13

[6] S-cerevisiae has three pathways for DNA interstrand crosslink repair [J].

Grossmann, KF ;

Ward, AM ;

Matkovic, ME ;

Folias, AE ;

Moses, RE .

MUTATION RESEARCH-DNA REPAIR, 2001, 487 (3-4) :73-83

[7] Role of PSO genes in the repair of photoinduced interstrand cross-links and photooxidative damage in the DNA of the yeast Saccharomyces cerevisiae [J].

Henriques, JAP ;

Brozmanova, J ;

Brendel, M .

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, 1997, 39 (03) :185-196

[8]

HENRIQUES JAP, 1980, GENETICS, V95, P273

[9] A founder mutation in Artemis, an SNM1-like protein, causes SCID in Athabascan-speaking Native Americans [J].

Li, LY ;

Moshous, D ;

Zhou, YG ;

Wang, JH ;

Xie, G ;

Salido, E ;

Hu, D ;

de Villartay, JP ;

Cowan, MJ .

JOURNAL OF IMMUNOLOGY, 2002, 168 (12) :6323-6329

[10] The β-lactamase motif in Snm1 is required for repair of DNA double-strand breaks caused by interstrand crosslinks in S-cerevisiae [J].

Li, XR ;

Moses, RE .

DNA REPAIR, 2003, 2 (01) :121-129