A carboxylated graphene and aptamer nanocomposite-based aptasensor for sensitive and specific detection of hemin

被引：31

作者：

Gao, Ling ^{[1
]}

Xiao, Yinghong ^{[1
]}

Wang, Yanping ^{[1
]}

Chen, Xue ^{[1
]}

Zhou, Bo ^{[1
]}

Yang, Xiaodi ^{[1
]}

机构：

[1] Nanjing Normal Univ, Coll Chem & Mat Sci, Jiangsu Collaborat Innovat Ctr Biomed Funct Mat, Jiangsu Key Lab New Power Batteries, Nanjing 210097, Jiangsu, Peoples R China

来源：

TALANTA | 2015年 / 132卷

基金：

中国国家自然科学基金; 高等学校博士学科点专项科研基金;

关键词：

Carboxylated grapheme; Functionalized electrode; Nanocomposite aptasensor; THROMBIN; CELLS; COMPOSITES; BIOSENSOR; ELECTRODE; CARBON; LABEL;

D O I：

10.1016/j.talanta.2014.09.010

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A unique nanocomposite was crafted by grafting hemin-binding-aptamer (HBA) onto carboxylated graphene (COO-GR). Infrared spectroscopy, Raman spectroscopy and diffuse reflectance spectra suggested that -NHCO- covalent bonds were formed between HBA and COO-GR. The resulting COO-GR/HBA functionalized electrode was used as a novel label-free biosensor. The square wave voltammetry was employed to realize the selective and specific detection of hemin. The obtained aptasensor possessed excellent performance with a detection limit of 0.64 nmol L-1 (S/N=3) and a linear range from 1 to 150 nmol L-1. Moreover, COO-GR was shown to be a promising candidate in making aptasensors, carrying advantages over graphene in terms of the simplicity of sensor preparation and the reduction of background noise. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：215 / 221

页数：7

共 28 条

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