Confocal imaging through weakly aberrating media

被引:10
作者
Sheppard, CJR [1 ]
机构
[1] Univ Sydney, Sch Phys, Dept Phys, Sydney, NSW 2006, Australia
[2] Univ Sydney, Australian Key Ctr Microscopy & Microanal, Sydney, NSW 2006, Australia
关键词
D O I
10.1364/AO.39.006366
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
The effect on confocal imaging of spherical aberration caused by a weak refractive-index mismatch is discussed. Aberration balancing that uses a change in the objective's tube length is studied. It is found that the range of depths that can be imaged satisfactorily by a high-numerical-aperture objective with compensation is an order of magnitude greater than that without compensation. The aberration balancing tends to break down for extremely high numerical apertures. (C) 2000 Optical Society of America. OCIS codes: 080.1010, 110.0180, 180.1790, 260.1960, 110.2990.
引用
收藏
页码:6366 / 6368
页数:3
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