Continuous microfluidic immunomagnetic cell separation

被引：297
作者：
Inglis, DW [1 ]
Riehn, R
Austin, RH
Sturm, JC
机构：
[1] Princeton Univ, Princeton Inst Sci & Technol, Dept Elect Engn, Princeton, NJ 08544 USA
[2] Princeton Univ, Dept Phys, Princeton, NJ 08544 USA
来源：
APPLIED PHYSICS LETTERS | 2004年 / 85卷 / 21期
基金：
美国国家卫生研究院;
关键词：
D O I：
10.1063/1.1823015
中图分类号：
O59 [应用物理学];
学科分类号：
摘要：
We present a continuous-flow microfluidic device that enables cell by cell separation of cells selectively tagged with magnetic nanoparticles. The cells flow over an array of microfabricated magnetic stripes, which create a series of high magnetic field gradients that trap the magnetically labeled cells and alter their flow direction. The process was observed in real time using a low power microscope. The device has been demonstrated by the separation of leukocytes from whole human blood. (C) 2004 American Institute of Physics.
引用
收藏
页码：5093 / 5095
页数：3
相关论文
共 9 条
  • [1] A fully integrated micromachined magnetic particle separator
    Ahn, CH
    Allen, MG
    Trimmer, W
    Jun, YN
    Erramilli, S
    [J]. JOURNAL OF MICROELECTROMECHANICAL SYSTEMS, 1996, 5 (03) : 151 - 158
  • [2] Stabilised cellular immuno-fluorescence assay: CD45 expression as a calibration standard for human leukocytes
    Bikoue, A
    Janossy, G
    Barnett, D
    [J]. JOURNAL OF IMMUNOLOGICAL METHODS, 2002, 266 (1-2) : 19 - 32
  • [3] Flow through, immunomagnetic cell separation
    Chalmers, JJ
    Zborowski, M
    Sun, LP
    Moore, L
    [J]. BIOTECHNOLOGY PROGRESS, 1998, 14 (01) : 141 - 148
  • [4] A new magnetic bead-based, filterless bio-separator with planar electromagnet surfaces for integrated bio-detection systems
    Choi, JW
    Ahn, CH
    Bhansali, S
    Henderson, HT
    [J]. SENSORS AND ACTUATORS B-CHEMICAL, 2000, 68 (1-3) : 34 - 39
  • [5] Hydrodynamics in 2-1/2 dimensions: making jets in a plane
    Darnton, N
    Bakajin, O
    Huang, R
    North, B
    Tegenfeldt, JO
    Cox, EC
    Sturm, J
    Austin, RH
    [J]. JOURNAL OF PHYSICS-CONDENSED MATTER, 2001, 13 (21) : 4891 - 4902
  • [6] KANTOR AB, 1998, MAGNETIC CELL SORTIN, P153
  • [7] Magnetic cell separation: Characterization of magnetophoretic mobility
    McCloskey, KE
    Chalmers, JJ
    Zborowski, M
    [J]. ANALYTICAL CHEMISTRY, 2003, 75 (24) : 6868 - 6874
  • [8] Prevention of protein adsorption by tethered poly(ethylene oxide) layers: Experiments and single-chain mean-field analysis
    McPherson, T
    Kidane, A
    Szleifer, I
    Park, K
    [J]. LANGMUIR, 1998, 14 (01) : 176 - 186
  • [9] HIGH-GRADIENT MAGNETIC CELL-SEPARATION WITH MACS
    MILTENYI, S
    MULLER, W
    WEICHEL, W
    RADBRUCH, A
    [J]. CYTOMETRY, 1990, 11 (02): : 231 - 238
1