Make microalgal cultures axenic again-a fast and simple workflow utilizing fluorescence-activated cell sorting

被引：11

作者：

Doppler, Philipp ^{[1
]}

Kriechbaum, Ricarda ^{[1
]}

Singer, Benedikt ^{[1
]}

Spadiut, Oliver ^{[1
]}

机构：

[1] Tech Univ Wien, Inst Chem Environm & Biosci Engn, Res Div Biochem Engn, Gumpendorfer Str 1a, A-1060 Vienna, Austria

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2021年 / 186卷

关键词：

Green algae; Cyanobacteria; Axenization; Isolation workflow; Chlorophyll fluorescence; FACS; SINGLE-CELL; FLOW-CYTOMETRY; BIOTECHNOLOGY; ESTABLISHMENT;

D O I：

10.1016/j.mimet.2021.106256

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Since the removal of contaminations in microalgal cultures is extremely laborious and time-consuming, we developed a rapid workflow to obtain axenicity by a combination of fluorescence-activated cell sorting (FACS) and plate spreading. During method development, several cyanobacteria and green algae strains were successfully made axenic. At the end, method transferability to another FACS device was demonstrated. Our workflow offers great time-savings with less hands-on laboratory work compared to conventional isolation techniques.

引用

页数：6

共 28 条

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Andersen RA, 2005, ALGAL CULTURING TECH, P83, DOI DOI 10.1016/B978-012088426-1/50007-X

[2]

[Anonymous], 2021, BOLTS BASAL MEDIUM B

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