Analysis of a challenging subset of world anti-doping agency-banned steroids and antiestrogens by LC-MS-MS

被引：22
作者：
Borges, Chad R. [1 ]
Miller, Nicole [1 ]
Shelby, Melinda [1 ]
Hansen, Megan [1 ]
White, Claudia [1 ]
Slawson, Matthew H. [1 ]
Monti, Kim [1 ]
Crouch, Dennis J. [1 ]
机构：
[1] Univ Utah, Sports Med Res & Testing Lab, Dept Pharmacol & Toxicol, Salt Lake City, UT 84108 USA
来源：
JOURNAL OF ANALYTICAL TOXICOLOGY | 2007年 / 31卷 / 03期
关键词：
D O I：
10.1093/jat/31.3.125
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A qualitative liquid chromatography-tandem mass spectrometry method for the analysis of 22 sporting federation-banned anabolic agents (or their metabolite markers) and anti-estrogens in urine that are refractory to analysis by gas chromatography-mass spectrometry is presented. In addition, a quantitative method built around World Anti-Doping Agency (WADA) guidelines for the confirmatory analysis of 19-norandrosterone, the primary metabolite of nandrolone with a WADA-specified minimum required performance limit of 1 ng/mL, is included. Hydrolysis of glucuronide conjugates, liquid-liquid extraction, no clean-up derivatization with Girard's Reagent P, and analysis by quadrupole-time-of-flight mass spectrometry provide sensitivity and selectivity well beyond that required by the WADA.
引用
收藏
页码：125 / 131
页数：7
相关论文
共 11 条
  • [1] Ege S, 1999, ORGANIC CHEM
  • [2] Geyer H., 1998, 15 COL WORKSH DOP AN, P99
  • [3] Analysis of oxysterols by electrospray tandem mass spectrometry
    Griffiths, WJ
    Wang, YQ
    Alvelius, G
    Liu, S
    Bodin, K
    Sjövall, J
    [J]. JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 2006, 17 (03) : 341 - 362
  • [4] Derivatisation for the characterisation of neutral oxosteroids by electrospray and matrix-assisted laser desorption/ionisation tandem mass spectrometry:: the Girard P derivative
    Griffiths, WJ
    Liu, S
    Alvelius, G
    Sjövall, J
    [J]. RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2003, 17 (09) : 924 - 935
  • [5] High-energy collision-induced dissociation of oxosteroids derivatised to Girard hydrazones
    Griffiths, WJ
    Alvelius, G
    Liu, S
    Sjövall, J
    [J]. EUROPEAN JOURNAL OF MASS SPECTROMETRY, 2004, 10 (01) : 63 - 88
  • [6] Huber L, 1999, VALIDATION QUALIFICA
  • [7] Ketosteroid profiling using Girard T derivatives and electrospray ionization tandem mass spectrometry: direct plasma analysis of androstenedione, 17-hydroxyprogesterone and cortisol
    Johnson, DW
    [J]. RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2005, 19 (02) : 193 - 200
  • [8] Analysis of derivatised steroids by matrix-assisted laser desorption/ionisation and post-source decay mass spectrometry
    Khan, MA
    Wang, YQ
    Heidelberger, S
    Alvelius, G
    Liu, SY
    Sjövall, J
    Griffiths, WJ
    [J]. STEROIDS, 2006, 71 (01) : 42 - 53
  • [9] Electrospray mass spectrometry of testosterone esters: Potential for use in doping control
    Shackleton, CHL
    Chuang, H
    Kim, J
    delaTorre, X
    Segura, J
    [J]. STEROIDS, 1997, 62 (07) : 523 - 529
  • [10] *WORLD ANT AG, WORLD ANT AG PROJ RE
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