Performance of Whole Blood Stimulation Assays for the Quantification of SARS-CoV-2 Specific T-Cell Response: A Cross-Sectional Study

被引：3

作者：

Bergami, Federica ^{[1
]}

Arena, Francesca ^{[1
]}

Pattonieri, Eleonora Francesca ^{[2
]}

Gregorini, Marilena ^{[2
]}

Meloni, Federica ^{[3
]}

Abelli, Massimo ^{[4
]}

Ticozzelli, Elena ^{[4
]}

Testa, Giorgia ^{[5
]}

Lilleri, Daniele ^{[1
]}

Cassaniti, Irene ^{[1
]}

Baldanti, Fausto ^{[1
,6
]}

机构：

[1] Fdn IRCCS Policlin San Matteo, Microbiol & Virol Unit, I-27100 Pavia, Italy

[2] Fdn IRCCS Policlin San Matteo, Nephrol Dialysis & Transplantat Unit, I-27100 Pavia, Italy

[3] Univ Pavia, Dept Internal Med & Med Therapeut, I-27100 Pavia, Italy

[4] Fdn IRCCS Policlin San Matteo, Gen Surg 4 Unit Abdominal Transplantat, I-27100 Pavia, Italy

[5] Fdn IRCCS Policlin San Matteo, Pediat Unit, I-27100 Pavia, Italy

[6] Univ Pavia, Dept Clin Surg Diagnost & Pediat Sci, I-27100 Pavia, Italy

来源：

DIAGNOSTICS | 2022年 / 12卷 / 06期

关键词：

COVID-19; T-cell response; IGRA; SARS CORONAVIRUS; COVID-19;

D O I：

10.3390/diagnostics12061509

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Since the identification of the new severe acute respiratory syndrome virus 2 (SARS-CoV-2), a huge effort in terms of diagnostic strategies has been deployed. To date, serological assays represent a valuable tool for the identification of recovered COVID-19 patients and for the monitoring of immune response elicited by vaccination. However, the role of T-cell response should be better clarified and simple and easy to perform assays should be routinely introduced. The main aim of this study was to compare a home-made assay for whole blood stimulation with a standardized ELISpot assay design in our laboratory for the assessment of spike-specific T-cell response in vaccinated subjects. Even if a good correlation between the assays was reported, a higher percentage of responder subjects was reported for immunocompromised subjects with ELISpot assay (56%) than home-made whole blood stimulation assay (33%). Additionally, three commercial assays were compared with our home-made assay, reporting a good agreement in terms of both positive and negative results.

引用

页数：7

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