Artificial Genetic Systems: Self-Avoiding DNA in PCR and Multiplexed PCR

被引：37

作者：

Hoshika, Shuichi ^{[1
]}

Chen, Fei ^{[1
]}

Leal, Nicole A. ^{[1
]}

Benner, Steven A. ^{[1
]}

机构：

[1] Fdn Appl Mol Evolut, Westheimer Inst Sci & Technol, Gainesville, FL 32601 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2010年 / 49卷 / 32期

关键词：

DNA analogues; DNA polymerases; polymerase chain reaction; pseudocomplementarity; synthetic biology; MODIFIED BASES; NUCLEIC-ACIDS; SEQUENCES; STABILITY; OLIGONUCLEOTIDES; HYBRIDIZATION; NUCLEOSIDES; INVASION; DUPLEXES;

D O I：

10.1002/anie.201001977

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Figure Presented. Discerning in their choice of partner: The DNA of a self-avoiding molecular-recognition system (SAMRS) binds to natural DNA but not to members of the same SAMRS. Multiplexed PCR with an SAMRS based on 2-aminopurine (A), 2-thiothymine (T*), 2'-hypoxanthine (C*). and N4ethylcytosine (C*; see examples) showed the benefits of such systems for clinical analysis, which requires many DNA molecules to interact with target DNA but not with each other. © 2010 Wiley-VCH Verlag GmbH &. Co. KGaA, Weinheim.

引用

页码：5554 / 5557

页数：4

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