Protein measurements of microalgal and cyanobacterial biomass

被引：452

作者：

Gonzalez Lopez, Cynthia Victoria ^{[1
]}

Ceron Garcia, Maria del Carmen ^{[1
]}

Acien Fernandez, Francisco Gabriel ^{[1
]}

Bustos, Cristina Segovia ^{[2
]}

Chisti, Yusuf ^{[3
]}

Fernandez Sevilla, Jose Maria ^{[1
]}

机构：

[1] Univ Almeria, Dept Chem Engn, E-04120 Almeria, Spain

[2] Fdn CAJAMAR, Estn Expt Las Palmerillas CAJAMAR, E-04710 Almeria, Spain

[3] Massey Univ, Sch Engn, Palmerston North, New Zealand

来源：

BIORESOURCE TECHNOLOGY | 2010年 / 101卷 / 19期

关键词：

Cyanobacteria; Kjeldahl method; Lowry method; Microalgae; Protein content; CONVERSION FACTORS; PHAEODACTYLUM-TRICORNUTUM; INTRACELLULAR NITROGEN; MARINE MICROALGAE; AMINO-ACIDS; GROWTH; QUANTIFICATION; ACCUMULATION; QUANTITATION; DISRUPTION;

D O I：

10.1016/j.biortech.2010.04.077

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

The protein content of dry biomass of the microalgae Porphyridium cruentum, Scenedesmus almeriensis, and Muriellopsis sp. and of the cyanobacteria Synechocystis aquatilis and Arthrospira platensis was measured by the Lowry method following disruption of the cells by milling with inert ceramic particles. The measurements were compared with the Kjeldahl method and by elemental analysis. The nitrogen-to-protein conversion factors for biomass obtained from exponentially growing cells with a steady state doubling time of similar to 23 h were 5.95 for nitrogen measured by Kjeldahl and 4.44 for total nitrogen measured by elemental analysis. The protein content in dry biomass ranged from 30% to 55%. The above conversion factors are useful for estimating the protein content of microalgal biomass produced in rapid steady state growth as encountered in many commercial production processes. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：7587 / 7591

页数：5

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