Comparison of Single-Copy and Multicopy Real-Time PCR Targets for Detection of Mycobacterium tuberculosis in Paraffin-Embedded Tissue

被引：26
|
作者：
Luo, Robert F. [1 ]
Scahill, Michael D. [2 ]
Banaei, Niaz [1 ,2 ]
机构：
[1] Stanford Univ, Sch Med, Dept Pathol, Stanford, CA 94305 USA
[2] Stanford Hosp & Clin, Clin Microbiol Lab, Palo Alto, CA 94304 USA
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 2010年 / 48卷 / 07期
关键词：
RAPID IDENTIFICATION; ASSAY; DIFFERENTIATION; MULTIPLEX; COMPLEX;
D O I：
10.1128/JCM.02449-09
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Real-time PCR can rapidly identify Mycobacterium tuberculosis in paraffin-embedded tissue in the absence of microbiological culture. In a comparison of single-copy and multicopy PCR targets in 70 tissue samples, the sensitivities were 26% and 54%, respectively, with 100% specificity. Sensitivity was 75% for newer samples and was not decreased for acid-fast bacillus (AFB) stain-negative specimens.
引用
收藏
页码：2569 / 2570
页数：2
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