Comparison of Single-Copy and Multicopy Real-Time PCR Targets for Detection of Mycobacterium tuberculosis in Paraffin-Embedded Tissue

被引：26
作者：
Luo, Robert F. [1 ]
Scahill, Michael D. [2 ]
Banaei, Niaz [1 ,2 ]
机构：
[1] Stanford Univ, Sch Med, Dept Pathol, Stanford, CA 94305 USA
[2] Stanford Hosp & Clin, Clin Microbiol Lab, Palo Alto, CA 94304 USA
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 2010年 / 48卷 / 07期
关键词：
RAPID IDENTIFICATION; ASSAY; DIFFERENTIATION; MULTIPLEX; COMPLEX;
D O I：
10.1128/JCM.02449-09
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Real-time PCR can rapidly identify Mycobacterium tuberculosis in paraffin-embedded tissue in the absence of microbiological culture. In a comparison of single-copy and multicopy PCR targets in 70 tissue samples, the sensitivities were 26% and 54%, respectively, with 100% specificity. Sensitivity was 75% for newer samples and was not decreased for acid-fast bacillus (AFB) stain-negative specimens.
引用
收藏
页码：2569 / 2570
页数：2
相关论文
共 13 条
[1]   Real-time quantitative PCR in the diagnosis of tuberculosis in formalin-fixed paraffin-embedded pleural tissue in patients from a high HIV endemic area [J].
Baba, Kamaldeen ;
Pathak, Sharad ;
Sviland, Lisbeth ;
Langeland, Nina ;
Hoosen, Anwar A. ;
Asjo, Birgitta ;
Dyrhol-Riise, Anne M. ;
Mustafa, Tehmina .
DIAGNOSTIC MOLECULAR PATHOLOGY, 2008, 17 (02) :112-117
[2]   EFFECTS OF TISSUE HANDLING AND PROCESSING STEPS ON PCR FOR DETECTION OF Mycobacterium tuberculosis IN FORMALIN-FIXED PARAFFIN-EMBEDDED SAMPLES [J].
Barcelos, Denise ;
Franco, Marcello F. ;
Leao, Sylvia Cardoso .
REVISTA DO INSTITUTO DE MEDICINA TROPICAL DE SAO PAULO, 2008, 50 (06) :321-326
[3]   Use of the real-time PCR assay in conjunction with MagNA pure for the detection of mycobacterial DNA from fixed specimens [J].
Beqaj, Safedin H. ;
Flesher, Randy ;
Walker, Ginar R. ;
Smith, Samuel A. .
DIAGNOSTIC MOLECULAR PATHOLOGY, 2007, 16 (03) :169-173
[4]  
Global Initiative for Asthma, 2009, PUBL WHO, V02-3659
[5]   Rapid Detection of Mycobacterium tuberculosis and Rifampin Resistance by Use of On-Demand, Near-Patient Technology [J].
Helb, Danica ;
Jones, Martin ;
Story, Elizabeth ;
Boehme, Catharina ;
Wallace, Ellen ;
Ho, Ken ;
Kop, JoAnn ;
Owens, Michelle R. ;
Rodgers, Richard ;
Banada, Padmapriya ;
Safi, Hassan ;
Blakemore, Robert ;
Lan, N. T. Ngoc ;
Jones-Lopez, Edward C. ;
Levi, Michael ;
Burday, Michele ;
Ayakaka, Irene ;
Mugerwa, Roy D. ;
McMillan, Bill ;
Winn-Deen, Emily ;
Christel, Lee ;
Dailey, Peter ;
Perkins, Mark D. ;
Persing, David H. ;
Alland, David .
JOURNAL OF CLINICAL MICROBIOLOGY, 2010, 48 (01) :229-237
[6]  
Hillemann D, 2006, INT J TUBERC LUNG D, V10, P340
[7]   Development of a real-time PCR-based method for rapid differential identification of Mycobacterium species [J].
Lim, S. Y. ;
Kim, B. -J. ;
Lee, M. -K. ;
Kim, K. .
LETTERS IN APPLIED MICROBIOLOGY, 2008, 46 (01) :101-106
[8]   Molecular differentiation of Mycobacterium tuberculosis strains without IS6110 insertions [J].
Lok, KH ;
Benjamin, WH ;
Kimerling, ME ;
Pruitt, V ;
Lathan, M ;
Razeq, J ;
Hooper, N ;
Cronin, W ;
Dunlap, NE .
EMERGING INFECTIOUS DISEASES, 2002, 8 (11) :1310-1313
[9]   PCR detection of Mycobacterium tuberculosis in necrotising non-granulomatous lymphadenitis using formalin-fixed paraffin-embedded tissue: a study in Thai patients [J].
Nopvichai, C. ;
Sanpavat, A. ;
Sawatdee, R. ;
Assanasen, T. ;
Wacharapluesadee, S. ;
Thorner, P. S. ;
Shuangshoti, S. .
JOURNAL OF CLINICAL PATHOLOGY, 2009, 62 (09) :812-815
[10]   Multiplex real-time PCR assay for rapid identification of Mycobacterium tuberculosis complex members to the species level [J].
Pinsky, Benjamin A. ;
Banaei, Niaz .
JOURNAL OF CLINICAL MICROBIOLOGY, 2008, 46 (07) :2241-2246
12