Comparison of Single-Copy and Multicopy Real-Time PCR Targets for Detection of Mycobacterium tuberculosis in Paraffin-Embedded Tissue

被引：26

作者：

Luo, Robert F. ^{[1
]}

Scahill, Michael D. ^{[2
]}

Banaei, Niaz ^{[1
,2
]}

机构：

[1] Stanford Univ, Sch Med, Dept Pathol, Stanford, CA 94305 USA

[2] Stanford Hosp & Clin, Clin Microbiol Lab, Palo Alto, CA 94304 USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2010年 / 48卷 / 07期

关键词：

RAPID IDENTIFICATION; ASSAY; DIFFERENTIATION; MULTIPLEX; COMPLEX;

D O I：

10.1128/JCM.02449-09

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Real-time PCR can rapidly identify Mycobacterium tuberculosis in paraffin-embedded tissue in the absence of microbiological culture. In a comparison of single-copy and multicopy PCR targets in 70 tissue samples, the sensitivities were 26% and 54%, respectively, with 100% specificity. Sensitivity was 75% for newer samples and was not decreased for acid-fast bacillus (AFB) stain-negative specimens.

引用

页码：2569 / 2570

页数：2

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