Study of effect of autacoids on water absorption and ion transport by skin of the frog Rana temporaria

The experiments on the frog Rana temporaria isolated skin showed participation of autacoids in regulation of the epithelium water permeability and of the transepithelial ion transport. The removal of autacoids secreted by the cells into the Ringer solution at its internal surface with the aid of frequent replacements of this solution leads to an increased water permeability and to a decreased transepithelial potential difference. Inhibition of prostaglandin synthesis with 1 x 10(-5) M indomethacin produces the frog skin depolarization. Addition of prostaglandin E-2 to the Ringer solution at the internal surface of the frog skin is accompanied by a decrease of the osmotic permeability, hyperpolarization, and an increase of short-circuit current. The non-contradictory model is described of the role of autacoids in regulation of the frog skin functions connected with participation of the skin in the water-salt homeostasis.