A thermo-responsive and photo-polymerizable chondroitin sulfate-based hydrogel for 3D printing applications

被引：104
作者：
Abbadessa, A. [1 ]
Blokzijl, M. M. [1 ,2 ]
Mouser, V. H. M. [2 ]
Marica, P. [1 ]
Malda, J. [2 ,3 ]
Hennink, W. E. [1 ]
Vermonden, T. [1 ]
机构：
[1] Univ Utrecht, Fac Sci, UIPS, Dept Pharmaceut, POB 80082, NL-3508 TB Utrecht, Netherlands
[2] Univ Med Ctr Utrecht, Dept Orthoped, POB 85500, NL-3508 GA Utrecht, Netherlands
[3] Univ Utrecht, Fac Vet Med, Dept Equine Sci, POB 80163, NL-3508 TD Utrecht, Netherlands
来源：
CARBOHYDRATE POLYMERS | 2016年 / 149卷
关键词：
Methacrylated chondroitin sulfate; Thermo-sensitive hydrogel; Photo-polymerization; Shear thinning; Cartilage 3D printing; MESENCHYMAL STEM-CELLS; HYALURONIC-ACID HYDROGELS; GLYCIDYL METHACRYLATE; HYPERBRANCHED POLYGLYCEROL; THERMOSENSITIVE HYDROGELS; EXTRACELLULAR-MATRIX; CHONDROGENESIS; DEGRADATION; SCAFFOLD; DEXTRAN;
D O I：
10.1016/j.carbpol.2016.04.080
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
The aim of this study was to design a hydrogel system based on methacrylated chondroitin sulfate (CSMA) and a thermo-sensitive poly(N-(2-hydroxypropyl) methacrylamide-mono/dilactate)-polyethylene glycol triblock copolymer (M15P10) as a suitable material for additive manufacturing of scaffolds. CSMA was synthesized by reaction of chondroitin sulfate with glycidyl methacrylate (GMA) in dimethylsulfoxide at 50 degrees C and its degree of methacrylation was tunable up to 48.5%, by changing reaction time and GMA feed. Unlike polymer solutions composed of CSMA alone (20% w/w), mixtures based on 2% w/w of CSMA and 18% of M15P10 showed strain-softening, thermo-sensitive and shear-thinning properties more pronounced than those found for polymer solutions based on M15P10 alone. Additionally, they displayed a yield stress of 19.2 +/- 7.0 Pa. The 3D printing of this hydrogel resulted in the generation of constructs with tailorable porosity and good handling properties. Finally, embedded chondrogenic cells remained viable and proliferating over a culture period of 6 days. The hydrogel described herein represents a promising biomaterial for cartilage 3D printing applications. (C) 2016 Elsevier Ltd. All rights reserved.
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收藏
页码：163 / 174
页数：12
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