Determination of Total Glutathione in Yeasts by High-Performance Liquid Chromatography with Dansylation

被引：0
作者：
Ma, Yunfeng [1 ]
Xiang, Fu [1 ]
Jin, Wenwen [1 ]
Yu, Longjiang [1 ]
机构：
[1] Huazhong Univ Sci & Technol, Coll Life Sci & Technol, Inst Resource Biol & Biotechnol, Wuhan 430074, Peoples R China
来源：
ZEITSCHRIFT FUR NATURFORSCHUNG SECTION C-A JOURNAL OF BIOSCIENCES | 2010年 / 65卷 / 5-6期
关键词：
Total Glutathione; Yeast; HPLC; AMINO-ACIDS; BLOOD; DERIVATIZATION; QUANTITATION; REDOX; CELLS;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A method to determine the content of total glutathione (GSHt) was introduced based on high-performance liquid chromatography (HPLC) with dansylation. The minimum detection concentration of GSHt was 0.5 mu g/mL and the measurable range 1.0-300 mu g/mL. GSHt in yeasts was obtained by hot-water extraction, GSH complete autoxidation to oxidized glutathione (GSSG) in alkaline solution and purification by thin-layer chromatography (TLC). The quantitative determination of GSSG was derived by dansyl chloride at pH 9.5, 60 degrees C for 60 min and assayed by HPLC. GSHt in Saccharomyces cerevisiae is higher than in Candida rugosa and Candida laths. S. cerevisiae can be chosen as the better target for mutagenesis and industrial scale.
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页码：391 / 394
页数：4
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