Carbon Nanotube-Bilirubin Oxidase Bioconjugate as a New Biofuel Cell Label for Self-Powered Immunosensor

被引：54
作者：
Cheng, Jiashun [1 ]
Han, Yajing [1 ]
Deng, Liu [1 ]
Guo, Shaojun [2 ]
机构：
[1] Cent S Univ, Coll Chem & Chem Engn, Changsha 410083, Hunan, Peoples R China
[2] Los Alamos Natl Lab, Los Alamos, NM 87545 USA
来源：
ANALYTICAL CHEMISTRY | 2014年 / 86卷 / 23期
基金：
中国博士后科学基金; 中国国家自然科学基金;
关键词：
GLUCOSE-OXIDASE; SENSOR; INHIBITION; BIOSENSOR;
D O I：
10.1021/ac503277w
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
We demonstrated a biofuel cells (BFCs)-based self-powered sensing system for the detection of N epsilon-(carboxymethyl)lysine (CML), in which the bilirubin oxidase (BOD)-carbon nanotube (CNT) bioconjugate modified with antibody acted as a biocatalyst for enhancing O-2 reduction in the biocathode, as well as the transducing enzyme for signaling magnification. With an increase in the concentration of CML, the amount of BOD labels on biocathode surface increases, thus leading to the higher output of the as-prepared BFCs. This novel BFCs-based self-powered sensor showed a wide linear range for analyzing CML from 1 nM to 100 mu M with a detection limit of 0.2 nM, which was 50 times more sensitive than that determined from the conventional ELISA. Most importantly, our new self-powered sensing platform can determine the level of CML in serum samples from multiple healthy donors and multiple sclerosis patients, being well in accordance with that from the commercial ELISA analysis.
引用
收藏
页码：11782 / 11788
页数：7
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