Three-dimensional super-resolution structured illumination microscopy with maximum a posteriori probability image estimation

被引：56

作者：

Lukes, Tomas ^{[1
,2
]}

Krizek, Pavel ^{[3
]}

Svindrych, Zdenek ^{[3
]}

Benda, Jakub ^{[4
]}

Ovesny, Martin ^{[3
]}

Fliegel, Karel ^{[1
]}

Klima, Milos ^{[1
]}

Hagen, Guy M. ^{[3
]}

机构：

[1] Czech Tech Univ, Fac Elect Engn, Dept Radioelect, Prague 16627 6, Czech Republic

[2] Ecole Polytech Fed Lausanne, Lab Opt Biomed LOB, CH-1015 Lausanne, Switzerland

[3] Charles Univ Prague, Fac Med 1, Inst Cellular Biol & Pathol, Prague 12801 2, Czech Republic

[4] Charles Univ Prague, Fac Math & Phys, Inst Theoret Phys, CR-18000 Prague, Czech Republic

来源：

OPTICS EXPRESS | 2014年 / 22卷 / 24期

关键词：

FIELD FLUORESCENCE MICROSCOPY; RESOLUTION LIMIT; CELLS; STORM; ARRAY; SOFI;

D O I：

10.1364/OE.22.029805

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We introduce and demonstrate a new high performance image reconstruction method for super-resolution structured illumination microscopy based on maximum a posteriori probability estimation (MAP-SIM). Imaging performance is demonstrated on a variety of fluorescent samples of different thickness, labeling density and noise levels. The method provides good suppression of out of focus light, improves spatial resolution, and allows reconstruction of both 2D and 3D images of cells even in the case of weak signals. The method can be used to process both optical sectioning and super-resolution structured illumination microscopy data to create high quality super-resolution images. (C) 2014 Optical Society of America

引用

页码：29805 / 29817

页数：13

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