DNA fluorescent labeling with naphtho[1,2,3-cd]indol-6(2H)-one for investigation of protein-DNA interactions

被引：3

作者：

Vasilyeva, Svetlana V. ^{[1
]}

Kuznetsov, Nikita A. ^{[1
]}

Kuznetsova, Anastasya S. ^{[2
]}

Khalyavina, Juliya G. ^{[2
]}

Tropina, Darya A. ^{[2
]}

Lavrikova, Tatyana I. ^{[2
]}

Kargina, Olga I. ^{[3
]}

Gornostaev, Leonid M. ^{[2
]}

机构：

[1] RAS, SB, Inst Chem Biol & Fundamental Med, Pr Lavrenteva 8, Novosibirsk 630090, Russia

[2] Astafev Krasnoyarsk State Pedag Univ, 89 Lebedev St, Krasnoyarsk 660049, Russia

[3] Ural State Med Univ, Repin St 3, Ekaterinburg 620028, Russia

来源：

BIOORGANIC CHEMISTRY | 2017年 / 72卷

基金：

俄罗斯基础研究基金会;

关键词：

Cu(I)-catalysed azide-alkyne cycloaddition; Click chemistry; Fluorescent azide; Fluorescently labeled DNA; Stopped-flow fluorescence study; Protein-DNA interactions; POSTSYNTHETIC FUNCTIONALIZATION; CLICK CHEMISTRY; RECOGNITION; REPAIR; KINETICS; ALKYNE; AZIDES;

D O I：

10.1016/j.bioorg.2017.05.002

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Fluorescently labeled DNA to study protein-DNA interactions was synthesized using the Cu(I)-catalysed cycloaddition (CuAAC) reaction. For this purpose, a new azido-containing fluorophore based on the naphtho[1,2,3-cd] indol-6(2H)-one derivative was obtained. The fluorescent properties of naphtho [1,2,3-cd] indol-6(2H)-one derivatives and labeled DNA were studied. The new fluorescent DNA conjugate was shown to be a useful tool to study complex mechanisms of protein-DNA interactions. (C) 2017 Elsevier Inc. All rights reserved.

引用

页码：268 / 272

页数：5

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