Dynamics and proliferative capacities of CD8+CD28+TCRαβ+CD62Lhigh T-cell subsets in healthy and asthmatic subjects

被引:1
作者
Pietruczuk, M. [1 ]
Kraszula, L. [1 ]
Kupczyk, M. [2 ]
Kuna, P. [2 ]
Eusebio, M. [1 ]
机构
[1] Med Univ Lodz, Dept Lab Diagnost, Lodz, Poland
[2] Med Univ Lodz, Dept Internal Med, Lodz, Poland
关键词
CD8(+)CD28(+) T cells; selectin; Foxp3; cytokines; anti-CD3/CD28; asthma; GROWTH-FACTOR-BETA; L-SELECTIN; DOWN-REGULATION; EXPRESSION; INHIBITION; CD62L; BLOOD; STIMULATION; LYMPHOCYTES; ACTIVATION;
D O I
暂无
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Adhesion molecules, as such, play essential roles in T-cell transendothelial extravasation during inflammation. A better understanding of the mechanisms underlying this process may be of value in the management of asthma. The present study employed Magnetic-Activated Cell Sorting (MACS) to isolate human CD8(+) T lymphocytes from peripheral blood of asthma patients and controls. The cells were flow cytometrically assessed to evaluate surface expression of an adhesion molecule, L-selectin (CD62L) on the surface of CD8(FoxP3-/bright T) cell subsets and its response to inflammatory cytokines. We showed that CD8(+)CD28(+)TCR alpha beta(+)CD62L(high)FoxP3(bright) T cells were deficient in blood of some asthma patients but abundant in others. After co-stimulation of CD8(+) T cells with anti-CD3/CD28 in combination with IL-2 and IL-10 or TGF-beta, the frequencies of CD8(+)CD28(+)TCR alpha/beta(+)CD62L(high) T cells in the group of patients were lower than at baseline. Our data indicate that L-selectin expression is regulated by inflammatory cytokines. Overall, these data reveal that asthma phenotypes may be further stratified into micro subtypes with distinct cellular and molecular characteristics, supporting the concept of asthma endotypes.
引用
收藏
页码:485 / 494
页数:10
相关论文
共 43 条
  • [1] IL-10 induces TGF-β secretion, TGF-β receptor II upregulation, and IgA secretion in B cells
    Bagheri, Yasser
    Babaha, Fateme
    Falak, Reza
    Yazdani, Reza
    Azizi, Gholamreza
    Sadri, Maryam
    Abolhassani, Hassan
    Shekarabi, Mehdi
    Aghamohammadi, Asghar
    [J]. EUROPEAN CYTOKINE NETWORK, 2019, 30 (03) : 107 - 113
  • [2] Oligoclonality of CD8+ T cells in health and disease: Aging, infection, or immune regulation?
    Batliwalla, F
    Monteiro, J
    Serrano, D
    Gregersen, PK
    [J]. HUMAN IMMUNOLOGY, 1996, 48 (1-2) : 68 - 76
  • [3] Loss of CD28 expression on CD8+ T cells is induced by IL-2 receptor γ chain signalling cytokines and type IIFN, and increases susceptibility to activation-induced apoptosis
    Borthwick, NJ
    Lowdell, M
    Salmon, M
    Akbar, AN
    [J]. INTERNATIONAL IMMUNOLOGY, 2000, 12 (07) : 1005 - 1013
  • [4] TGFβ Programs Central Memory Differentiation in Ex Vivo-Stimulated Human T Cells
    Dahmani, Amina
    Janelle, Valerie
    Carli, Cedric
    Richaud, Manon
    Lamarche, Caroline
    Khalili, Myriam
    Goupil, Mathieu
    Bezverbnaya, Ksenia
    Bramson, Jonathan L.
    Delisle, Jean-Sebastien
    [J]. CANCER IMMUNOLOGY RESEARCH, 2019, 7 (09) : 1426 - 1439
  • [5] Increasing JAK/STAT Signaling Function of Infant CD4+ T Cells during the First Year of Life
    dela Pena-Ponce, Myra Grace
    Rodriguez-Nieves, Jennifer
    Bernhardt, Janice
    Tuck, Ryan
    Choudhary, Neelima
    Mengual, Michael
    Mollan, Katie R.
    Hudgens, Michael G.
    Peter-Wohl, Sigal
    De Paris, Kristina
    [J]. FRONTIERS IN PEDIATRICS, 2017, 5
  • [6] Enhanced apoptosis of T cells in common variable immunodeficiency (CVID): role of defective CD28 co-stimulation
    Di Renzo, M
    Zhou, Z
    George, I
    Becker, K
    Cunningham-Rundles, C
    [J]. CLINICAL AND EXPERIMENTAL IMMUNOLOGY, 2000, 120 (03) : 503 - 511
  • [7] PREVENTION OF IN-VITRO NEUTROPHIL-ENDOTHELIAL ATTACHMENT THROUGH SHEDDING OF L-SELECTIN BY NONSTEROIDAL ANTIINFLAMMATORY DRUGS
    DIAZGONZALEZ, F
    GONZALEZALVARO, I
    CAMPANERO, MR
    MOLLINEDO, F
    DELPOZO, MA
    MUNOZ, C
    PIVEL, JP
    SANCHEZMADRID, F
    [J]. JOURNAL OF CLINICAL INVESTIGATION, 1995, 95 (04) : 1756 - 1765
  • [8] Lung function decline in asthma: association with inhaled corticosteroids, smoking and sex
    Dijkstra, A
    Vonk, JM
    Jongepier, H
    Koppelman, GH
    Schouten, JP
    ten Hacken, NHT
    Timens, W
    Postma, DS
    [J]. THORAX, 2006, 61 (02) : 105 - 110
  • [9] Defining Airflow Obstruction
    Eschenbacher, William L.
    [J]. CHRONIC OBSTRUCTIVE PULMONARY DISEASES-JOURNAL OF THE COPD FOUNDATION, 2016, 3 (02): : 515 - 518
  • [10] Eusebio M, 2013, J BIOL REG HOMEOS AG, V27, P681