Effects of disturbed flow on endothelial cells

被引:165
作者
Chiu, JJ [1 ]
Wang, DL
Chien, S
Skalak, R
Usami, S
机构
[1] Acad Sinica, Inst Biomed Sci, Taipei 11529, Taiwan
[2] Univ Calif San Diego, Dept Bioengn, La Jolla, CA 92093 USA
[3] Univ Calif San Diego, Inst Biomed Engn, La Jolla, CA 92093 USA
来源
JOURNAL OF BIOMECHANICAL ENGINEERING-TRANSACTIONS OF THE ASME | 1998年 / 120卷 / 01期
关键词
D O I
10.1115/1.2834303
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Atherosclerotic lesions tend to localize at curvatures and branches of the arterial system, where the local flow is often disturbed and irregular (e.g., flow separation, recirculation, complex flow patterns, and nonuniform shear stress distributions). The effects of such flow conditions on cultured human umbilical vein endothelial cells (HUVECs) were studied in vitro by using a vertical-step flow channel (VSF). Detailed shear stress distributions and flow structures have been computed by using the finite volume method in a general curvilinear Coordinate system. HUVECs in the reattachment areas with low shear stresses were generally rounded in shape. fn contrast, the cells under higher shear stresses were significantly elongated and aligned with the flow direction, even far those in the area with reversed flow. When HUVECs were subjected to shearing in VSF, their actin stress fibers reorganized in association with the morphological changes. The rate of DNA synthesis in the vicinity of the flow reattachment area was higher than that in the laminar flow area. These in vitro experiments have provided data for the understanding of the in vivo responses of endothelial cells under complex flow environments found in regions of prevalence of atherosclerotic lesions.
引用
收藏
页码:2 / 8
页数:7
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