Molding a Silver Nanoparticle Template on Polydimethylsiloxane to Efficiently Capture Mammalian Cells

被引：19

作者：

Bai, Hai-Jing ^{[1
]}

Gou, Hong-Lei ^{[1
]}

Xu, Jing-Juan ^{[1
]}

Chen, Hong-Yuan ^{[1
]}

机构：

[1] Nanjing Univ, Sch Chem & Chem Engn, Key Lab Analyt Chem Life Sci, Nanjing 210093, Peoples R China

来源：

LANGMUIR | 2010年 / 26卷 / 04期

基金：

中国国家自然科学基金;

关键词：

ANTIBACTERIAL ACTIVITY; SURFACE MODIFICATION; URINARY CATHETERS; POLY(DIMETHYLSILOXANE); MICROPATTERNS; LITHOGRAPHY; FABRICATION; PROTEIN; FILMS; PDMS;

D O I：

10.1021/la902683x

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Herein, a functional template made up of in situ synthesized silver nanoparticles (AgNPs) is prepared on polydimethylsiloxane (PDMS) for the spatial control of cell capture, where the residual Si-H groups in the PDMS matrix are used as reductants to reduce AgNO3 for forming AgNPs. In virtue Of microfluidic system, a one-dimensional array pattern of AgNPs is obtained easily. Further combining with plasma treatment, a two-dimensional array pattern of AgNPs could be achieved. The obtained PDMS-AgNPs composite is characterized in detail. The PDMS-AgNPs composite shows good antibacterial property in E. coli adhesion tests. The patterns possess hifi and high resolution (ca. Spill). Cell patterns with high efficiency and spatial selectivity are Further formed with the aid of H-Arg-Gly-Asp-Cys-OH (RGDC) tetrapeptide which is grafted oil the AgNPs template. Cells immobilized oil the template show it good ability for adhesion, spreading, migration, and growth.

引用

页码：2924 / 2929

页数：6

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