Two forms of RNA polymerase II holoenzyme display different abundance during the cell cycle

被引：2
|
作者：
Holland, L [1 ]
Yankulov, K [1 ]
机构：
[1] Univ Guelph, Dept Mol Biol & Genet, Guelph, ON N1G 2W1, Canada
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2003年 / 302卷 / 03期
关键词：
RNA polymerase II holoenzyme; cell cycle;
D O I：
10.1016/S0006-291X(03)00214-6
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We analyzed the composition and abundance of two forms of RNA polymerase 11 (pol 11) holoenzyme in synchronized HeLa cells. We did not detect significant changes in pol 11 holoenzyme composition, but we noticed differences in the abundance of the two complexes at different stages of the cell cycle. Summarized data from several independent experiments demonstrate that pol 11 holoenzyme, which is purified by GST-TFIIS affinity chromatography, is more abundant during G1/S and S phases. Another form of pol 11 holoenzyme, which is purified by anti-CDK7 antibodies, shows relatively higher amounts in G2/M and early G1 phases. (C) 2003 Elsevier Science (USA). All rights reserved.
引用
收藏
页码：484 / 488
页数：5
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